蓚酸カルシウム結石患者赤血球膜におけるoxalate influx rateの検討

Abstract

対象は再発性蓚酸カルシウム結石患者群10名, 健康対照群18名である.1)陰イオン交換系阻害剤であるDIDSの存在下では赤血球内への蓚酸輸送は大きく阻害され, RBC oxalate influxはband 3 proteinを介した現象であると考えられた.2) RBC oxalate influx rateに影響を与える因子は反応系の温度, 反応系のpHであり, 反応温度を0°C, その時のpHを7.4とした条件下でRBC oxalate influx rateの測定が可能であった.3)健常対照群および蓚酸カルシウム結石患者でRBC oxalate influx rateを測定した結果, 有意差を認め, 尿路結石患者の一部には体細胞膜での蓚酸輸送異常の存在する可能性も考えられた

We established a method of human red blood cell oxalate influx rate under the condition of steady state exchange. Using this method we measured the influx rate in 10 patients with recurrent calcium oxalate nephrolithiasis and in 18 controls. DIDS inhibited oxalate flux across the human red blood cell membrane. This result suggested that band 3 protein mediates oxalate transport. Oxalate influx rate depended on reaction temperature and pH of reaction buffer. Although the oxalate influx rate in 4 degrees C could not be determined under an initial condition rate, the measurable condition was in 0 degrees C of reaction temperature. Consequently we measured the oxalate influx rate under the condition of 0 degrees C reaction temperature and 20 ml volume of washing buffer. The mean oxalate influx rate was significantly higher in patients with nephrolithiasis than in controls (-1.00 +/- 0.19 vs. -0.78 +/- 0.14).