シクロスポリン腎毒性における腎組織血流量の変化

Abstract

実験には体重約100 gのWistar系成熟雄ラットを使用した.実験群は, Cs投与群(Cs群)と対照群の2群とし, 動物数は各群7匹とした.Cs群の各ラットには, 1日体重1 kg当り50 mgのCsを10日間連日腹腔内投与した.対照群の各ラットには, 溶媒として用いた生理食塩水(生食)だけを10日間連日腹腔内投与した.Cs投与により, 腎皮質の組織血流量は減少した.この原因として, 小葉間動脈より末梢の細い血管系に変化が起き, 末梢血管抵抗が上昇した可能性が考えられた.Cs投与でRAA系が活性化されることから, 障害が生ずるのは糸球体の輸入細動脈である可能性が大きい

To elucidate the relationship between cyclosporine (Cs) nephrotoxicity and renal blood flow (RBF), we carried our experiments using rats. Adult male Wistar strain rats each weighing about 100 g were used. Rats were divided into 2 groups; the Cs group (Cs 50 mg/kg/day was intraperitoneally given for 10 consecutive days) and the control group (normal saline solution for 10 consecutive days). The renal cortical blood flow was measured in each group by electrolytic hydrogen gas clearance method. We also carried out renal angiography with barium sulfate (BaSO4). Then we measured blood vessel area/renal sagittal section area ratio and the diameter of interlobar arteries. The Cs group showed a significant decrease of renal cortical blood flow compared with the control group. On the other hand there was no significant change in renal angiogram. In conclusion, Cs decreased renal cortical blood flow. We suppose that vascular resistance increased following afferent arteriolopathy caused by Cs administration.