尿路結石症におけるInvitroの結晶形成

Abstract

蓚酸カルシウム結晶形成, 成長, 凝集の検討を主としてCoulter counterを用いた.1) seed crystal法, 全尿系でCG-120, SPPに阻止効果を認めた.2) CG-120, SPPがcontinuous crystallizer systemでnucleation rateを抑制した.3) ESWL後の結石片を持続結晶作製装置内におき成長させる系を開発しCG-120の溶解効果を検討し, 結石片成長阻止効果を認めた.4)ラットに蓚酸カルシウム結晶を誘発させin vivoにおいてもSPPに結晶成長, 凝集阻止効果が認められた

The formation, growth and aggregation of calcium oxalate crystals were evaluated with a Coulter counter. CG-120 and sodium pentosan polysulfate (SPP) has inhibitory activity on calcium oxalate crystal formation, growth and aggregation in the seeded crystal system and whole urine system. In continuous crystallizer system, they inhibit the nucleation rate. A new system of observing the crystal formation and growth of fragmented stones after extracorporeal shock wave lithotripsy has been developed. CG-120 has an inhibitory effect on their growth. The deposition of calcium oxalate crystals in the rat kidney was induced, and the, the volume and number of crystals were estimated using a Coulter counter. SPP has an inhibitory effect on calcium oxalate crystal growth in vivo.