ヒト前立腺組織特異抗原の研究 : 未知の抗原に対するモノクローナル抗体作製法のモデル

Abstract

前立腺分泌細胞, 基底細胞, 間質細胞などの混合物を免疫原にし, BPH切片に対する蛍光抗体法でスクリーニングしてマウスモノクローナル抗体を作製した。前立腺分泌細胞に良く反応し, ホルマリン固定パラフィン切片にも適用可能である, PAP, PSAにそれぞれ特異的な抗体を産生する細胞を1クローンずつ樹立できた

Mouse monoclonal antibodies were prepared using a cell mixture containing prostatic secretory, basal cells and stromal cells as an immunogen, and by the immunofluorescence method with cryosections of prostate tissue. An immunohistochemical screening process was used in the expectation that it would help identify not only the antibodies reacting with the secretory cells predominant in the prostate but also those reacting with relatively rare cells in the prostate tissue. Two cell clones have been established which produce antibodies (IgG1) that react well with prostatic secretory cells: a clone producing antibodies specific to prostatic acid phosphatase (PAP) and one producing antibodies specific to prostate-specific antigen (PSA). These antibodies could recognize the native antigens in the seminal plasma, respectively. These findings indicate that antibodies recognizing only one substance can be prepared, even when a mixture of prostatic tissue including many proteases is used as an immunogen. Production of antibodies against prostate-specific antigens other than PAP and PSA by this method should provide a useful means for analyzing unknown antigens in the prostate.