特発性男子不妊症精巣の組織化学的研究 : 精細管壁における酸性複合糖質の検討

Abstract

1)正常および乏精子症精巣の精細管壁に分布する酸性複合糖質分子種はCHS-A, BおよびCであった。2)無精子症精巣の精細管壁に分布する酸性複合糖質分子種はCHS-Bが主体があった。3)特発性男子不妊症精巣の造精能障害は精細管の壁の肥厚による単純な栄養障害のほかに, 精細管の壁に含まれる酸性複合糖質分子種の相違による機能的な相違も密接に関連しているものと考えられた

Acidic glycoconjugates in the seminiferous tubular walls in the testes from patients with idiopathic male infertility was identified light microscopically with the sensitized high iron diamine method in combination with digestions with chondroitinase ABC, chondroitinase B or testicular hyaluronidase. Tissue specimens were obtained by testicular biopsy from 37 patients with idiopathic male infertility and 9 fertile adult males. Chondroitin sulfate A, B and C were identified in the tubular walls of oligozoospermic patients with idiopathic male infertility irrespective of the thickness of the walls. Similar results were obtained in the tubular walls of the testes from normal males. On the other hand, chondroitin sulfate B was a main acidic glycoconjugate in the tubular walls of the testes from azoospermic patients with idiopathic male infertility irrespective of the thickness of the walls. These findings suggest that the etiological factors of the impaired spermatogenesis in patients with idiopathic male infertility are not only the disturbance of nutritional transport across the seminiferous tubular walls due to peritubular thickening but the functional alterations of the tubular walls associated with changes in components of acidic glycoconjugates in the tubular walls. The pathogenesis of oligozoospermia does not seem to be similar to that of azoosspermia since components of acidic glycoconjugates in the peritubular tissues between the two types of disorders are quite different.