ダウンロード数: 220
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
30_1405.pdf | 688.02 kB | Adobe PDF | 見る/開く |
タイトル: | 蓚酸カルシウム結石患者における血中蓚酸濃度について |
その他のタイトル: | Plasma oxalate concentration in calcium oxalate stone formers |
著者: | 保科, 彰 ![]() |
著者名の別形: | HOSHINA, Akira |
キーワード: | Calcium oxalate urolithiasis Plasma oxalate concentration Urinary oxalate Spinach load Renal oxalate clearance |
発行日: | Oct-1984 |
出版者: | 泌尿器科紀要刊行会 |
誌名: | 泌尿器科紀要 |
巻: | 30 |
号: | 10 |
開始ページ: | 1405 |
終了ページ: | 1415 |
抄録: | gas chromatograph法で少量の試料で血中蓚酸定量を可能とした.結石(S)群31例, 対照(C)群16例の空腹時蓚酸血中濃度(POA)はそれぞれ1.45±0.39, 1.37±0.39 pg/mlで, 有意差ではない.ホウレン草100 g負荷後のPOAはS群5例では2 h後より, S群5例では4 h後より有意に上昇し, 6 h後のS群は232%, C群は167%と上昇した.S群の2, 4, 6 h値はC群より有意に高かった.ホウレン草負荷後1~2 h尿中排泄量は負荷前に比しS群282%, C群250%と有意に増加し, C群では2 h後に排泄ピークを, S群は4~6 h後あるいはそれ以後にみた.S群4例とC群6例の蓚酸クリアランスは19.0, 18.0 ml/分で, 有意差はなかった.クレアチニンクリアランとの比は両群ともに0.22であった.S群はC群に比し, 蓚酸に対する吸収能力が亢進しているのみでなく, 吸収部位も拡大していると考えられ, 尿中への排泄は遅延していると考えた A sensitive, simplified method for plasma oxalate determination by gas chromatography is described. After deproteinizing the plasma with 3N HC1 and 20% sulfosalicylic acid, the oxalate was methylated, extracted and analysed by gas chromatography. This method has three advantages i.e., smaller sample size (plasma 5.0 ml), rapidity (takes less than 3 hours) and accuracy. The recovery rate of oxalate added to plasma was 91.42 +/- 11.31% (SD) and the coefficient of variation of replicate determinations was 4.18%. The minimum detectable concentration of oxalate was 0.3 micrograms/ml (oxalate peak was higher than 5 mm). The mean oxalate concentration under fasting conditions from 16 healthy subjects was 1.37 +/- 0.39 micrograms/ml (SD), while that from 31 calcium oxalate stone formers was 1.45 +/- 0.39 micrograms/ml (SD). There was no significant difference in plasma oxalate concentration between the two groups. The dietary influence of oxalate on plasma and urinary oxalate was investigated in 5 healthy subjects and 5 calcium oxalate stone formers. When 100 g spinach (total oxalate 545.5 mg, soluble oxalate 381.5 mg) was given, the increase of plasma oxalate concentration was more prominent in stone formers; in stone formers it increased to 142% of control value at 2 hours (p less than 0.05) after spinach loading, to 163% at 4 hour (p less than 0.01) and to 232% at 6 hours (p less than 0.01); while in healthy subjects increased to 119% at 2 hours (ns) after loading, to 144% at 4 hours (p less than 0.05) and only to 167% at 6 hours (p less than 0.01). Urinary oxalate excretion increased promptly between 1 and 2 hours after loading in both groups, reaching peak levels between 2 and 4 hours after loading in healthy subjects and between 4 and 6 hours or later in stone formers. The mean renal clearance of oxalate was 18.0 ml/min in 6 healthy subjects and 19.0 ml/min in 4 calcium oxalate stone formers. There was no significant difference in oxalate clearance between the two groups. The mean ratio of oxalate/creatinine clearance was 0.22 for stone formers, which was equal to that for healthy subjects. |
URI: | http://hdl.handle.net/2433/118306 |
PubMed ID: | 6524552 |
出現コレクション: | Vol.30 No.10 |
![](/dspace/image/articlelinker.gif)
このリポジトリに保管されているアイテムはすべて著作権により保護されています。