Enzyme immunoassay法による前立腺性酸性フォスファターゼの検討

Abstract

We measured prostatic acid phosphatase levels by enzymeimmunoassay (PAP-EIA). Intraassay reproducibility of PAP-EIA was markedly good. In thirty normal males, PAP-EIA levels ranged from 0.24 ng/ml to 3.3 ng/ml, mean and S.D. being 0.94 ng/ml, 0.50 ng/ml, respectively. We made the upper limit of PAP-EIA for the normal range, 1.94 ng/ml (Mean + 2 S.D.). O 40 patients with untreated prostatic cancer, 34 patients (85%) gave positive results (1/3 33% of stage A, 3/4 75% B, 10/11 90% C, 20/22 90% D). One out of 12 patients with BPH, and one out of 11 with prostatitis gave positive results. The false positive rate was 9%. PAP levels of 324 samples from 111 patients with prostatic cancer were measured by EIA and radioimmunoassay (RIA). A significant correlation was noted between EIA and RIA (r = 0.997 p less than 0.001).