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タイトル: | 新しい酵素法によるポリアミン測定について 第6報: 血中総ポリアミン定量法の基礎的検討および尿路性器癌患者における臨床的検討 |
その他のタイトル: | Detection of polyamines by an enzymatic assay. (6). Fundamental and clinical studies of a simple enzymatic method for determining total polyamines in blood |
著者: | 小出, 卓也 酒井, 俊助 嶋津, 良一 鄭, 漢彬 原, 明 沢田, 英夫 |
著者名の別形: | KOIDE, Takuya SAKAI, Shunsuke SHIMAZU, Ryoichi TEI, Kanhin HARA, Akira SAWADA, Hideo |
キーワード: | Total polyamines in blood A new enzymatic method |
発行日: | May-1987 |
出版者: | 泌尿器科紀要刊行会 |
誌名: | 泌尿器科紀要 |
巻: | 33 |
号: | 5 |
開始ページ: | 645 |
終了ページ: | 650 |
抄録: | A simple enzymatic method for determining total polyamines in human blood was established. Polyamines in trichloroacetic acid extract from 1 ml of blood were isolated on an anion-exchange column and measured spectrophotometrically by the end point assay using polyamine oxidase and putrescine oxidase. The recovery was as high as 98.4% and within-run precision (coefficient of variance: 1.82%), and the values obtained by this method were in fair agreement with those obtained by high performance liquid chromatography and the enzymatic differential assay methods previously reported. The polyamine levels were not changed when the blood was stored at -20 degrees C. Blood polyamine levels were measured in 108 patients with genitourinary cancers, 29 patients with benign prostatic hypertrophy, 18 patients with benign urological diseases and 25 normal subjects. Although polyamines were not significantly elevated in the low stage of cancer, elevation was observed at a high stage of malignancy. These results indicate that the determination of total polyamines may be useful to determine biochemically the malignancy of cancers. |
URI: | http://hdl.handle.net/2433/119145 |
PubMed ID: | 3661330 |
出現コレクション: | Vol.33 No.5 |
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