水欠乏性脱水の腎機能への影響に関する実験的研究

Abstract

SD系雄ラットを2, 4, 5日間完全脱水にし, 腎機能, 水素ガスクリアランス法による腎組織血流量および腎組織のadenine nucleotideを測定した.1)水欠乏性脱水時の腎機能は正常に保たれていた.2)水素ガスクリアランス法による腎組織血流量の測定の結果, 脱水の進行とともに腎髄質血流が有意に減少し(p<0.01), 腎皮質血流量は若干増加した.このときの平均動脈圧は腎灌流圧の自己調節領域下限であった.3)腎組織のATP値はA群(完全脱水2日間)で低下し, B(同4日間)・C(同5日間)群で若干増加したが, 腎組織のEC (Energy charge)は正常に保たれていた

To clarify the renal function induced by water deprivation-induced dehydration, intrarenal blood flow and changes in adenine nucleotide level in the renal tissue were investigated. Male SD rats were completely deprived of water for 2, 4 and 5 days. The intrarenal blood flow was determined by the hydrogen-gas clearance method and adenine nucleotide level in the renal tissue by high pressure liquid chromatography (HPLC) on the 2nd, 4th, and 5th day after water deprivation. The renal function and energy charge (EC = [ATP + 1/2 ADP]/[ATP + ADP + AMP]) were normal in all rats. In the dehydrated rats, the urine and blood were concentrated, and the medullary blood flow decreased. The mean arterial blood pressure was in the lower limit of renal perfusion pressure under autoregulation. In conclusion, the lower limit of renal perfusion pressure under autoregulation and urine and blood concentration due to water deprivation-induced dehydration may easily cause acute renal failure.