前立腺疾患におけるγ-Seminoproteinの臨床的研究 第2報:γ-Seminoproteinの免疫組織化学的検討

Abstract

1)酵素抗体直接法にて前立腺肥大症全例において, 腺上皮細胞内にγ-Smの局在を認めた.2)原発性前立腺癌以外の悪性腫瘍においては, γ-Smの組織内局在は認められなかった.3)前立腺癌については, γ-Smの組織内局在は53%に認められ, 組織の分化度とγ-Smの局在陽性率に関して正の相関傾向が認められた.4) γ-Smの組織内局在と血清γ-Sm値間には明らかな関係を見出せなかった

Localization of gamma-seminoprotein (gamma-Sm) was examined using horseradish peroxidase-labeled anti-gamma-Sm antibody (Chugai Corp. Ltd., Tokyo, Japan) by the enzyme-labeled antibody method in paraffin embedded specimens of 18 benign prostatic hyperplasias and 32 untreated prostatic cancers. The level of serum gamma-Sm was also determined by enzyme immunoassay in 10 untreated patients with prostatic cancer and 18 with benign prostatic hyperplasia. Specific gamma-Sm staining was detected in prostatic glandular epithelial cells and prostatic secretion of all specimens of benign prostatic hyperplasias and a half of the specimens of prostatic cancers. Specific gamma-Sm staining was shown to correlate with histological differentiation of the prostatic cancer, but no correlation was found between specific gamma-Sm staining and the level of serum gamma-Sm.