膀胱腫瘍のレクチン結合糖鎖

Abstract

1)表在性膀胱腫瘍40例, 浸潤性膀胱腫瘍18例を用いて, レクチンによる染色性と再発・grade・深達度との関係を検討した.2)非再発例および表在性再発例では, DBA, WGA陽性, Con A陰性であった.3) Grade 1, 2ではDBA, BS-1の陽性率が高く, grade 3ではCon Aの陽性率が高かった.4) TaおよびT1とT2以上を比較した場合, 低深達度のものは, DBA, WGAが高い陽性率をしめし, 高深達度のものはCon Aとの結合が多かった.5)以上より, WGA, DBAによる染色性の喪失とCon Aによる染色性は, 悪性度の増加にともう変化であると推測した

We studied the lectin binding patterns of 40 initial superficial and 10 subsequent invasive bladder tumors by the avidin-biotin-peroxidase complex (ABC) method using the following biotin-labeled lectins: PNA, DBA, UEA-I, BS-I, ConA and WGA. We observed the relationship between lectin binding and subsequent course of initial superficial tumors, grade and stage (T). DBA or WGA staining tumors and Con A negative tumors revealed no recurrence or superficial recurrence. Low grade tumors were DBA or BS-I positive and high grade tumors were ConA positive. Low staging tumors possessed DBA or WGA positiveness and high staging tumors had ConA positiveness. From these results we considered that negative staining of WGA or DBA, or positive staining of ConA was a change accompanying the malignant potentiality.