膀胱癌患者における細胞性免疫能の検討 : 加齢と膀胱癌細胞T24に対するリンパ球細胞障害性

Abstract

膀胱癌細胞T24を標的細胞とする細胞性免疫能の検討を, 健常者39例, 膀胱癌42例, 非膀胱腫瘍泌尿器癌15例の3群で行った.1) T24細胞に対する, 正常人NK活性は加齢により低下する傾向があった.2)膀胱癌群では, 加齢による細胞障害性の低下は認められず, 高齢者間の3群の比較では, 疾患関連性を有する細胞性免疫能の存在を示唆する結果を得た.3)膀胱癌症例に認められたT24細胞に対する細胞障害性にも, NK細胞の関与が強く示唆された.4)膀胱癌群に認められた細胞障害性は, stage間の比較では, low stage群で高値を示す傾向があった.grade別の比較では差異がなかった

The cytotoxic activity of peripheral blood lymphocytes of 39 control subjects, 42 bladder tumor patients and 15 patients with urological cancer other than bladder tumor against human bladder tumor cultured cell T24 was tested by a 51Cr-release assay. In the healthy donor group, high cytotoxic activity was found in younger people and negative correlation was significantly found between the cytotoxicity and age. On the other hand, high cytotoxic activity was frequently found in older patients with bladder tumors and no correlation between the cytotoxicity and age was found in this group. The mean % cytotoxicity for patients over 50 years old in the bladder tumor group was significantly higher than that for the healthy donor group and other carcinoma group. A large proportion of the cytotoxicity of the control subjects and the bladder tumor patients to T24 was due to lymphocytes that did not form spontaneous rosettes with sheep erythrocytes.