ヒト膀胱癌由来培養細胞株KK-47に対する抗癌剤と温熱の併用殺細胞効果

Abstract

ヒト膀胱癌由来培養細胞KK-47を用い, in vitroにおけるadriamycinおよびpeplomycinの殺細胞効果, 42°Cもしくは43°C温熱の併用による殺細胞効果を検討した.1)この細胞の生残率を20%もしくは50%阻止するために必要な加温時間をIT20, およびIT50とした場合に, 42°C IT20, 43°C IT20, および43°C IT50値はそれぞれ41, 22, 41分であった.2) Adriamycin単独では細胞生存率曲線上, 濃度依存性に細胞生残率の低下が認められ, 温熱を併用した場合には抗癌剤濃度が0.02 μg/ml以下の場合には殺細胞効果の増大が認められたが, 抗癌剤濃度が0.05 μg/ml以上の場合には, 抗癌剤単独群との殺細胞効果の差は減少した.3) Peplomycinは細胞生残率曲線上, 上に凹の2～3相性のカーブを示し, 温熱の併用では細胞生残率曲線上での軽度の傾きの増大が認められた

Using an in vitro colony forming assay system, cytotoxic effects of anticancer drugs, adriamycin (ADM) and peplomycin (PEP), and the combined effect of hyperthermia and anticancer drugs on cultivated KK-47 cells were investigated. From the response curves obtained at 42 and 43 degrees C hyperthermia, 20% growth inhibition time (IT20) at 42 and 43 degrees C hyperthermia and 50% growth inhibition time (IT50) at 43 degrees C were calculated. The IT20 and IT50 hyperthermia were combined with a 2-hour treatment of each of the anticancer drugs. When the hyperthermia was combined with various concentrations of ADM ranging from 0.005 to 0.1 microgram/ml, enhanced cell killing effects were obtained at the concentrations of less than 0.02 microgram/ml of ADM, whereas, there was no increase in cell killing effect at the concentrations of more than 0.05 microgram/ml of ADM. The combination of hyperthermia with PEP considerably enhanced the cell killing effects with an increase of PEP concentration.