Mitomycin C大量衝撃療法が著効を示した陰茎癌の1症例 組織学的およびオートラジオグラフィーによる経時的観察

Abstract

Administration of a massive dose (1 mg/kg) of mitomycin C attempted to a 73-year-old man suffering from penile cancer with metastases to the both inguinal lymph nodes resulted in a remarkable effect. Biopsy was performed before the therapy and 24 hours, 10 days and 20 days after the administration of mitomycin C. The specimens were immediately placed in a solution contained tritiated thymidine in tissue culture medium, and incubated at 3TC for 2 hours. Then the tissues were fixed in a 10 % neutral formalin for 24 hours. At the end of fixation, the tissue sections were made for routine histological examinations with H & E stain, as well as autoradiographic studies by means of stripping film method with exposure for 28 days at 4°C. The results obtained are summarized as follows. 1) Before the administration of mitomycin C: The H & E stain section showed squamous cell carcinoma. Autoradiograms on the same tissue disclosed high DNA synthesis with the findings of 40~50 grains per cell and 4.1 % labeling index. 2) 24 hours after the administration of mitomycin C : The H & E stained section revealed moderate alterations of the tumor cells and round cell infiltration. The radioautogram showed moderate quality of DNA synthesis, 40±grains per cell, with the labeling index of 4.1 '36. 3) 10 days after the treatment: Degenerative changes of the tumor cells, especially in their nuclei, was distinctly demonstrated. A shrinkage of the nuclei ''Vas observed with more intense basophilic stainability. Karyolysis and giant cell formations were also seen. The tumor tissue showed marked round cell infiltration. The grade of 3H-thymidine incorporation was low, showing 30±grains per cell. The labeling index of the same tissue was 2.4 %. 4) 20 days after the treatment: The lesion histologically consisted of necrotic tissue with fibrosis and cellular infiltration. No labeled cell was recognized.