副睾丸に関する研究 第1報 : 実験的急性副睾丸炎の作製に関する研究

Abstract

E. coli, Ps. aeruginosa, Pr. mirabilisおよびS. marcescensを用い実験的副睾丸炎を作製した.1) 3 kg前後の雄家兎の精管のみ露出し, 睾丸に向って精管内に108, 106, 105, 104, 103, cells/mlとなるように調整した菌液0.1 mlを注入し, 注入後3日目に屠殺した.2)実験的副睾丸炎の発生は, 肉眼的, 組織学的に, Ps. aeruginosaがもっとも強く, Pr. mirabilis, S. marcescens, E. coliの順に炎症反応が強かった.3)副睾丸炎の強弱の程度をみるのにpoint counting methodを利用し副睾丸管々腔の拡張の程度から菌種, 菌量などの関連を観察した.Ps. aeruginosaを使用した群でもっとも管腔拡大が認められ, ついでPr. mirabilis, S. marcescens, E. coliの順であった.4)急性副睾丸炎時には, 睾丸にも炎症性変化のほかにspermatogenesisの低下が認められた.5)副睾丸組織中の細菌学的検討では, 組織学的に高度な変化を起すPs. aeruginosa, Pr. mirabilis, S. marcescensでは副睾丸の各部位で菌が検出されたのに対し, E. coliでは菌を検出できないものが多かった

Nonspecific epididymitis is not an uncommon disease, but few reports on it were found in a search of the literature. The present paper reports an investigation that was undertaken to elucidate the mechanism of development of nonspecific epididymitis using an experimental model. Material and methods: Rabbits each weighing about 3 kg were used in the experiment. Following intravenous anesthetization, an incision of about 1 cm was made into the skin in the inguinal region to expose only the ductus deferens, and 0.1 ml of inoculum containing 10[8], 10[6] , 10[5] , 10[4] or 10[3] cells/ml of clinically isolated Ps. aeruginosa A 14, S. marcescens No 2, Pr.mirabilis GN 79 or E. coli C 11 strains was injected into the ductus deferens near the tail of the epididymis in the direction of the testes. At 3 days after inoculation, the animals were sacrificed, and the ductus deferens, epididymis, and testicle were removed for bacteriological and histological studies. Results: All the strains of microorganisms tested caused epididymitis. The affected organs or tissues were examined bacteriologically and histologically: Ps. aeruginosa had caused the severest infection, followed by Pro mirabilis, S. marcescens, and E. coli in decreasing order. E. coli, however, at an inoculum size of 10[8] cells/ml or smaller barely caused infection. The dilated lumens of affected epididymiis were measured by the point counting method. Epididymal dilation was found to correlate closely with the histological findings of epididymal infection. With the progTess of epididymitis, an infectious disease in the testes and diminished spermatogenesis were also noted. Epididymal tissue was examined for causative microorganisms. In the groups exposed to Ps. aeruginosa, Pro mirabilis or S. marcescens which cause severe infection, all sites examined were positive for these strains of organisms, whereas the groups receiving E. coli inocula of 10[4] cells/ml or less were completely negative.