代償性腎肥大におけるDNA合成およびmitotic activityについて

Abstract

Hyperplasia of the renal parenchyma, one of the factors of compensatory hypertrophy of the kidney, was investigated by determining intracellular DNA with microspectrophotometry as well as by determining mitotic rate of the each anatomical fraction of the kidney after administration of colchicine. 1) After uninephrectomy, weight of the remaining kidney showed increase of 25% on the 14th day, and at that time, it was still increasing. 2) DNA synthesis of the remaining kidney after uninephrectomy, revealed presence of the cells of synthetic resting stage already on the first day. The highest activity was seen on the 5th day, when approximately 6 % of cells were at synthetic resting stage. This was striking in the cortex. No such cells were found two weeks after nephrectomy. 3) The highest mitotic activity was seen on the 5 th day with monophasic curve. The cortex was again remarkable in this activity, whereas the inner medullary zone showed almost no activity. 4) DNA synthesis correlated well with mitotic activity through the course of experimental days.