膀胱癌患者におけるβ-Glucuronidaseの研究

Abstract

The finding of a relationship between bladder carcinoma and β-glucuronidase (β-G) in 1947 arose the attention of many investigators. Thus a number of papers have reported the fact that a β-G activity in urine is high among patients with bladder carcinoma. It is, however, not yet clear what the main course of these observations is. The present biochemical studies were aimed to find the relationship between the increase of β-G activity in urine of bladder carcinoma patients and their tumors. The data are summarized as follows: 1. The levels of β-G activity in both urine and serum of bladder carcinoma patients were higher than those of the normal subjects. The β-G activity of the extract of bladder carcinoma was also higher than that of the normal. The β-G activity of the kidney extract was the highest value in the normal subject, but there were no significant difference between the activity of kidney extract and that of bladder carcinoma statistically. 2. By Pevicon block electrophoresis two bands from the bladder carcinoma extract and one band from the normal bladder extract were observed. 3. By DEAE-cellulose column chromatography the β-G activity of the bladder carcinoma extract emerged as three peaks designated as Fractions I, II and III, in contrast to one peak (Fr. I) from the normal bladder and two peaks (Fr. I and III) from kidney were detected. Fr. II was observed only in the bladder carcinoma extract. 4. By DEAE-cellulose column chromatography the β-G activity of urine of bladder carcinoma patients was fractionated into three fractions (Fr. I, II and III) in contrast to two fractions (Fr. I and III) of that of normal urine. Fr. II was also specific for urine of the bladder carcinoma patients, likewise in the tissue extract. 5. These data suggested that the β-G activity in urine of the bladder carcinoma patient is apparently derived from the carcinoma tissue.