ヒト腎細胞癌の基礎的研究 第2報: 腎細胞癌細織内および患者血清中アルカリフォスファターゼアイソザイムについて

Abstract

A fundamental study was performed about alkaline phosphatase (orthophosphoric monoester phosphohydrorase (E.C., 3.1.3.1.) activity and its isozyme patterns in 4 normal renal tissues, 24 renal cell carcinoma tissues and sera from patients with the tumor. The results obtained were as follows. 1) Alkaline phcsphatase activity in four normal renal tissues was rather consistent and the isozyme pattern in polyacrylamide gel disk electrophoresis consisted of 3 or 4 components. 2) Alkaline phosphatase was not detected from renal cell carcinoma tissue in three cases. The other twenty-one had the activity of various degrees. The activity was rather high in well differentiated renal cell carcinoma compaired with poorly differentiated ones, although the difference was not statistically significant. With respect to the cell type, i.e. clear, granular or mixed cell type, there was no correlation with the activity of the enzyme. 3) Renal cell carcinoma tissue was found to contain at least a component of alkaline phosphatase isozymes of kidney proper except a case with bone type and two cases with liver type alkaline phosphatase. In addition to this, several other isozymes were detected including Kasahara isozyme, fetal intestinal type alkaline phosphatase and two isozymes of unknown characteristics. 4) Including 3 cases with elevated serum alkaline phosphatase activity without any evidence of liver or bone metastasis, serum alkaline phosphatase isozyme in renal cell carcinoma patients was regarded as the liver type. These results rendered it difficult to support the modern concept of histogenesis of renal cell carcinoma stating that this carcinoma is developed exclusively from the proximal convoluted tubules. Further investigations will be needed for the establishment of this concept. The elevated serum alkaline phosphatase in patients with renal cell carcinoma was thought to be mainly hepatic origin as described by several authors.