膀胱のvinding detense mechanismの数量的解析 4: 滲透圧およびpHのgeneration timeにおよぼす影響について

Abstract

An experiment was carried out in order to investigate the effects of osmotic pressure and hydrogen ion concentration on the generation time of various bacteria. The following result was obtained. 1) E. coli, Serratia, Klebsiella, Rettgerella and Pseudomonas aeruginosa were isolated from the urine specimens of patients with urinary tract infection. A strain of each bacteria was cultured in the two systems, one being the broth of pH 7.0 with the osmotic pressure 100, 300, 500, 700 and 900 mOsm/liter and another that of 296 mOsm/liter with the hydrogen ion concentration pH 5, 6, 7, 8 and 9. The generation time was measured. Osmotic pressure gave little effect, but pH gave the considerable effect on the generation time of E. coli, Rettgerella and Pseudomonas aeruginosa which was prolonged in pH 5.0. 2) In order to know these effects more precisely, two factors, pH and osmotic pressure, were given three different levels. Three levels in the former were pH 5.5, 7.0 and 8.5 and that in the latter was 150, 300 and 600 mOsm/litre. Thus, two-repetition method was carried out on three levels and two factors. The order of 18 measurements was completely randomized. The results obtained was analysed according to Duncan's range test at the risk level of 5%. Significant difference was observed in the generation time of Klebsiella, Rettgerella and Pseudomonas aeruginosa at the pH of acid side and that of Pseudomonas aeruginosa at the low osmotic pressure as well as at the high osmotic pressure.