抗菌剤の膀胱腔内への移行にかんする研究 1.両腎摘除ラットにおける各種抗菌剤の血清ならびに膀胱内生理的食塩水中濃度について

Abstract

In order to obtain the basic data regarding the prevention and treatment of infection of the defunctionalized bladder, an investigation was made to see if the antibacterial agent administered generally is transfered into the intravesical space. Male rats, weighing 300 to 350 grams, were nephrectomized bilaterally and their penis was ligated. The bladder was instilled with 0.7 ml of normal saline. This was designated as the intravesical fluid. Various antibacterial agents were administered to these rats which were sacrificed 24, 43, and 72 hours after that in order to determine the serum and intravesical fluid concentration of the agents. In amino glycoside group such as kanamycin, DKB and gentamicin, the serum concentration showed a relatively high level even at 72 hours following the gradual decline. The intravesical fluid concentration showed the peak level at 48 hours and the almost same level with the serum at 72 hours. In rifampicin and nalidixic acid, the serum concentration decreased in the course of time. Antibacterial activity was proved in the 72 hours serum in rifampicin and 48 hours serum in nalidixic acid. Intravesical fluid also showed small activities. In aminobenzyl-penicillin, sulbenicillin and cefamezin, tiny activity was proved in the serum and intravesical fluid. Aminobenzyl-penicillin or sulbenicillin disappeared from the serum and cefamezin from the intravesical fluid in the early period of the experiment. In vitro stability of aminobenzyl-penicillin, cefamezin, kanamycin and oleandomycin in the intravesical fluid was examined. In aminobenzyl-penicillin and cefamezin, contaminated bacteria achieving growth during the experiment might have enhanced the inactivation. The activity was not affected by the change of pH.