抗癌剤の経膀胱的吸収に関する研究 -膀胱内注入療法の基礎- 第2報：3H-Adriamycinの経膀胱的吸収,体内分布,排泄について

Abstract

The absorption of 3H-adriamycin through rat urinary bladder and the subsequent distribution and excretion of the absorbed adriamycin were studied. Male Wistar rats weighing 300 to 350 g were used. A ureter was exposed and half-incised transversely, using a stereoscopic microscope. A polyethylene tube with an outer diameter of 0.024 inches was inserted through this incision, in the same manner as an ureterostomy, and the opposite kidney was extirpated. As for the bile, the same tube was inserted into the bile duct. Then, 3H-adriamycin (10.79µCi/0.3 ml/300µg adriamycin) was injected into the bladder. Following the instillation of the adriamycin over an one hour period, the filled bladder was excised. Thereafter the urine and bile were collected every 3 hours for the next 12 hours. In another group, the liver, spleen, kidney and heart were extirpated I and 12 hours after the injection, respectively. The following results were obtained. 1) The recovery rate of the residual 3H-adriamycin in bladder one hour after instillation was 81.7% and the vesical tissue level was 175.9µg/g on an average. 2) The tissue concentrations of 3H-adriamycin one hour after the injection were 0.19µg//g in the liver, 0.36, ug/g in the spleen, 0.31µg/g in the kidney, and 0.21µg//g in the heart. 3) The recovery rate of 3H-adriamycin into the urine was 0.66%, and the rate of it into bile was 4.22% during 12 hours. 4) The tissue concentrations of 3H-adriamycin 12 hours after the injection were 0.52µg/g in the liver, 0.67µg/g in the spleen, 0.49µg/g in the kidney and 0.33µg/g in the heart. Adriamycin is well absorbed through bladder, and then distributed to various organs, particularly liver, spleen, kidney and heart. The absorbed adriamycin is very slowly excreted into bile and urine.