膀胱癌再発防止法としてのCarboquoneとUrokinaseの併用膀胱内注入療法 -第1報-

Abstract

The cytocidal effect of 6 anticancer agents on in vitro cultivated KK-47 cells, established from a human bladder carcinoma, was determined by a colony forming method. Carboquone showed the most remarkablc cytotoxic effects in terms of the assessment of 50 percent lethal dose in the 2-, and 24-hour exposures of anticancer agents; 3.5×10-3 and 1.1×[-3]µg/ml. A combined exposure of urokinase (10 CTA u./ml), and carboquone (2.0×10[-3]µg/ml) resulted in a significant decrease of cell growth as compared with the single exposure of carboquone. In addition, [3]H-thymidine uptakes of the cells were remarkably reduced by the combination exposure. Based on the results obtained in the experimental studies, a combined intravesical instillation therapy of carboquone and urokinase for the prevention of recurrence of bladder carcinoma was performed in 17 patients. One of the 17 patients showed a recurrence of bladder carcinoma. The recurrence rate for 14 months after operation was 6.3 percent using an actuarial method. No significant side effect was observed. The combined instillation therapy was considered a potent useful one for the prevention of recurrence of bladder carcinoma.