DRL-917キットによる前立腺酸性フォスファターゼ測定法の検討

Abstract

Prostatic acid phosphatase (PAP) concentrations were measured by radioimmunoassay (RIA) kit (DRL-917). 1) The coefficients of variance of PAP concentrations in inter-assay and intra-assay were 9.81±4.45% and 7.89±2.45%. 2) The recovery rates in dilution test and recovery test were 98.75±7.93% and 97.05±7.43%. 3) Significant correlation (r=0.687, p<0.01) was seen between enzymatic assay (Bessey-Lowry method) and RIA (PAP concentrations> 2.0 ng/ml). 4) There was no sex difference of PAP concentrations in normal adults, such as 0.417 ±0.411 ng/ml (n=52) in normal males and 0.435±0.577 ng/ml (n=10) in normal females, and the correlation between PAP concentration and aging was not significant. 5) All of the untreated patients and 65.5% of the treated patients with prostatic cancer of stage D had higher PAP concentrations than 2.0 ng/ml in sera, and RIA of PAP seemed to be more specific to prostatic cancer than enzymatic assay in which 91.6% and 23.3% of those had increased activities. But PAP concentrations were elevated falsely in 2 out of 11 patients with benign prostatic hyperplasia.