RIA法による前立腺性酸フォスファターゼの診断的価値

Abstract

The measurement of human prostatic acid phosphatase (PAP) was studied using PAP RIA KIT which was first established in Japan by Eiken Immunochemical Laboratory. The immunological activity of PAP was stable for 24 hours at room temperature and had no cross-reactivity with erythrocytes. In the clinical phase, PAP was measured in the serum obtained from 37 patients with benign prostatic hypertrophy, 47 patients with treated and untreated prostatic cancer, 43 patients with other urological diseases and 31 volunteers of healthy adult men. From this result, it was concluded that the normal range of PAP was less than 3.19ng/ml. In the group of untreated prostatic cancer, the positivity of PAP by radioimmunoassay was 78% in stage III and 100% in stage IV. In both stages, it was higher than that of enzymatic assay. False positive by radioimmunoassay was 5 to 10%, and false negative 14%. As conclusion the method of radioimmunoassay for measurement of PAP is much more reliable than enzymatic assay.