ヒト腎細胞癌の基礎的研究 第6報 : 培養系での腎癌細胞と正常腎細胞の各種抗癌剤に対する感受性の相異について

Abstract

To find out more effective chemotherapeutic agents against human renal carcinomas, in vitro sensitivity test of 12 drugs (cyclophosphoamide, thio-TEPA, carbazilquinone, ACNU, 5-fluorouracil, methotrexate, cytosine arabinoside, mitomycin C, adriamycin, bleomycin, vinblastine and cis-DDP) were carried out on an established renal carcinoma cell line, OUR-10, and the results were compaired with the sensitivity of in vitro cultivated human normal renal epithelial cells. The cellular damage caused by the drugs were estimated by the inhibition of intracellular incorporation of 3H-thymidine or 14C-leucine. The inhibition of the isotopic incorporation into normal kidney cells was almost equal or significantly higher compaired with that into the cancer cells except for mitomycin C, ACNU and 5-fluorouracil. Methotrexate was a curious drug causing the more enhanced thymidine incorporation but the more reduced leucine incorporation into OUR-10 cells. Several problems included in the present experiment were outlined. But reviewing the past clinical results concerning about the chemotherapy on human renal carcinomas, vinblastine, mitomycin C, 5-fluorouracil and hydroxyurea were the rather effective drugs in comparison with others, the sensitivity test herein described was considered to be an useful aid seeking improved chemotherapy on human renal carcinomas.