実験的神経因性膀胱作成ラットにおけるacetylcholinesterase活性の検討

Abstract

Experimental study of the neurogenic bladder was carried out on female rats. Neurogenic bladder was induced by 10% phenol glycerin block of the spinal cord. The time course of acetylcholinesterase activity was investigated both chemically and histochemically, throughout the postcperative period of one to 28 days studied. This method is available as model of clinical picture for 7 to 14 days after operation. The content of acetylcholinesterase activity decreased rapidly during the 2 days following operation and finally reached approximately 30% of initial level after 7 days. But at 28 days after operation, restoration of acetylcholinesterase activity and staining were presented. On the other hand cholinesterase activity declined only to about 50% of its activity on initial level during the 2 days, and it seldom went below 60%. The reduction of acetylcholinesterase staining observed in the rat neurogenic bladder was paralleled the fall in acetylcholinesterase activity determined chemically.