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j.antiviral.2008.05.006.pdf | 299.39 kB | Adobe PDF | 見る/開く |
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DCフィールド | 値 | 言語 |
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dc.contributor.author | Nishikawa, Hiroki | en |
dc.contributor.author | Kodama, Eiichi | en |
dc.contributor.author | Sakakibara, Ayako | en |
dc.contributor.author | Fukudome, Ayako | en |
dc.contributor.author | Izumi, Kazuki | en |
dc.contributor.author | Oishi, Shinya | en |
dc.contributor.author | Fujii, Nobutaka | en |
dc.contributor.author | Matsuoka, Masao | en |
dc.contributor.alternative | 大石, 真也 | ja |
dc.contributor.alternative | 児玉, 栄一 | ja |
dc.date.accessioned | 2011-02-16T05:14:45Z | - |
dc.date.available | 2011-02-16T05:14:45Z | - |
dc.date.issued | 2008-10 | - |
dc.identifier.issn | 0166-3542 | - |
dc.identifier.uri | http://hdl.handle.net/2433/137211 | - |
dc.description.abstract | Entry of human immunodeficiency virus type 1 (HIV-1) into target cells is mediated by its envelope protein gp41 through membrane fusion. Interaction of two extra-virion heptad repeats (HRs) in the gp41 plays a pivotal role in the fusion, and its inhibitor, enfuvirtide (T-20), blocks HIV-1 entry. To identify agents that block HIV-1 fusion, two screening methods based on detection and quantification by the enzyme-linked immunosorbent assay (ELISA) principle have been established. One method uses an alkaline phosphatase (ALP)-conjugated antibody (Ab-ELISA) and the other uses an ALP-fused HR (F-ELISA) to detect and quantify the interaction of the two HRs. The F-ELISA was more simple and rapid, since no ALP-conjugated antibody reaction was required. Both ELISAs detected all the fusion inhibitors tested except for T-20. Interaction of the two HRs was observed in both ELISAs, even in the presence of 10% dimethyl sulfoxide. Ab-ELISA performed best in a pH ranging from 6 to 8, while F-ELISA performed best at a pH ranging from 7 to 8. These results indicate that both established ELISAs are suitable for the identification of HIV-1 fusion inhibitors. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier BV | en |
dc.rights | © 2008 Elsevier B.V. | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.subject | HIV-1 | en |
dc.subject | Gp41 | en |
dc.subject | Fusion | en |
dc.subject | ELISA | en |
dc.subject | Screening | en |
dc.subject | Alkaline phosphatase | en |
dc.subject.mesh | Alkaline Phosphatase/chemistry | en |
dc.subject.mesh | Amino Acid Sequence | en |
dc.subject.mesh | Drug Evaluation, Preclinical/methods | en |
dc.subject.mesh | Enzyme-Linked Immunosorbent Assay/methods | en |
dc.subject.mesh | HIV Envelope Protein gp41/chemistry | en |
dc.subject.mesh | HIV Envelope Protein gp41/metabolism | en |
dc.subject.mesh | HIV Fusion Inhibitors/chemical synthesis | en |
dc.subject.mesh | HIV Fusion Inhibitors/chemistry | en |
dc.subject.mesh | HIV Fusion Inhibitors/pharmacology | en |
dc.subject.mesh | HIV-1/drug effects | en |
dc.subject.mesh | HIV-1/pathogenicity | en |
dc.subject.mesh | Humans | en |
dc.subject.mesh | Membrane Fusion/drug effects | en |
dc.subject.mesh | Repetitive Sequences, Amino Acid/genetics | en |
dc.subject.mesh | Virion/chemistry | en |
dc.title | Novel screening systems for HIV-1 fusion mediated by two extra-virion heptad repeats of gp41. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA10623661 | - |
dc.identifier.jtitle | Antiviral research | en |
dc.identifier.volume | 80 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 71 | - |
dc.identifier.epage | 76 | - |
dc.relation.doi | 10.1016/j.antiviral.2008.05.006 | - |
dc.textversion | author | - |
dc.identifier.pmid | 18584890 | - |
dcterms.accessRights | open access | - |
dc.identifier.pissn | 0166-3542 | - |
出現コレクション: | 学術雑誌掲載論文等 |
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