このアイテムのアクセス数: 137

このアイテムのファイル:
ファイル 記述 サイズフォーマット 
journal.pone.0005540.pdf955.43 kBAdobe PDF見る/開く
タイトル: Intra-platform repeatability and inter-platform comparability of microRNA microarray technology.
著者: Sato, Fumiaki
Tsuchiya, Soken
Terasawa, Kazuya
Tsujimoto, Gozoh
著者名の別形: 佐藤, 史顕
発行日: May-2009
出版者: Public Library of Science(PLoS)
誌名: PloS one
巻: 4
号: 5
論文番号: e5540
抄録: Over the last decade, DNA microarray technology has provided a great contribution to the life sciences. The MicroArray Quality Control (MAQC) project demonstrated the way to analyze the expression microarray. Recently, microarray technology has been utilized to analyze a comprehensive microRNA expression profiling. Currently, several platforms of microRNA microarray chips are commercially available. Thus, we compared repeatability and comparability of five different microRNA microarray platforms (Agilent, Ambion, Exiqon, Invitrogen and Toray) using 309 microRNAs probes, and the Taqman microRNA system using 142 microRNA probes. This study demonstrated that microRNA microarray has high intra-platform repeatability and comparability to quantitative RT-PCR of microRNA. Among the five platforms, Agilent and Toray array showed relatively better performances than the others. However, the current lineup of commercially available microRNA microarray systems fails to show good inter-platform concordance, probably because of lack of an adequate normalization method and severe divergence in stringency of detection call criteria between different platforms. This study provided the basic information about the performance and the problems specific to the current microRNA microarray systems.
著作権等: © 2009 Sato et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
URI: http://hdl.handle.net/2433/138085
DOI(出版社版): 10.1371/journal.pone.0005540
PubMed ID: 19436744
出現コレクション:学術雑誌掲載論文等

アイテムの詳細レコードを表示する

Export to RefWorks


出力フォーマット 


このリポジトリに保管されているアイテムはすべて著作権により保護されています。