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タイトル: | In vitro proliferation and chondrogenic differentiation of rat bone marrow stem cells cultured with gelatin hydrogel microspheres for TGF-beta1 release. |
著者: | Ogawa, Toshihiro Akazawa, Toshiyuki Tabata, Yasuhiko ![]() |
著者名の別形: | 田畑, 泰彦 |
キーワード: | MSC PROLIFERATION CHONDROGENIC DIFFERENTIATION TGF-β1 RELEASE MICROSPHERES SCAFFOLD |
発行日: | 1-Mar-2010 |
出版者: | VSP, an imprint of Brill |
誌名: | Journal of biomaterials science. Polymer edition |
巻: | 21 |
号: | 5 |
開始ページ: | 609 |
終了ページ: | 621 |
抄録: | The objective of this study was to evaluate the proliferation and chondrogenic differentiation of rat bone marrow-derived mesenchymal stem cells (MSCs) cultured with gelatin hydrogel microspheres of cell scaffold which can release transforming growth factor-beta1 (TGF-beta1). Gelatin was dehydrothermally cross-linked in different conditions in a water-in-oil emulsion state to obtain gelatin hydrogel microspheres with different water content. The microspheres functioned not only as the scaffold of MSC, but also the carrier matrix of TGF-beta1 release. The MSC proliferation depended on the water content of microspheres. Higher MSC proliferation was observed for the gelatin microspheres with lower water content. When cultured with the gelatin hydrogel microspheres, MSC formed their aggregates, in contrast to culturing with hydrogel sheets. The cell viability was significantly high compared with that of the hydrogel sheet. The production of sulfated glycosaminaglycan (sGAG) from MSC was examined as a measure of chondrogenic differentiation, after their culturing in a normal and chondrogenic differentiation media. For both the cultures, the amount of sGAG produced was significantly higher for MSC cultured with the gelatin microspheres than that of the gelatin sheet. Stronger differentiation of MSC was achieved in culture with the microspheres incorporating TGF-beta1 than that of MSC cultured in the medium containing the same amount of TGF-beta1. It is concluded that the gelatin hydrogel microspheres function well as both the scaffold of MSC and the matrix of TGF-beta1 release, resulting in enhanced MSC aggregation and the consequent promotion of cell proliferation and differentiation. |
著作権等: | © 2010 VSP, an imprint of Brill |
URI: | http://hdl.handle.net/2433/139430 |
DOI(出版社版): | 10.1163/156856209X434638 |
PubMed ID: | 20338095 |
出現コレクション: | 学術雑誌掲載論文等 |
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