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DCフィールド | 値 | 言語 |
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dc.contributor.author | Ogawa, Jun | en |
dc.contributor.author | Kodera, Tomohiro | en |
dc.contributor.author | Smirnov, Sergey V | en |
dc.contributor.author | Hibi, Makoto | en |
dc.contributor.author | Samsonova, Natalia N | en |
dc.contributor.author | Koyama, Ryoukichi | en |
dc.contributor.author | Yamanaka, Hiroyuki | en |
dc.contributor.author | Mano, Junichi | en |
dc.contributor.author | Kawashima, Takashi | en |
dc.contributor.author | Yokozeki, Kenzo | en |
dc.contributor.author | Shimizu, Sakayu | en |
dc.contributor.alternative | 小川, 順 | ja |
dc.contributor.alternative | 日比, 慎 | ja |
dc.date.accessioned | 2011-05-10T00:51:20Z | - |
dc.date.available | 2011-05-10T00:51:20Z | - |
dc.date.issued | 2011-03 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.uri | http://hdl.handle.net/2433/139589 | - |
dc.description.abstract | 4-Hydroxyisoleucine (HIL) found in fenugreek seeds has insulinotropic and anti-obesity effects and is expected to be a novel orally active drug for insulin-independent diabetes. Here, we show that the newly isolated strain Bacillus thuringiensis 2e2 and the closely related strain B. thuringiensis ATCC 35646 operate a novel metabolic pathway for L-isoleucine (L-Ile) via HIL and 2-amino-3-methyl-4-ketopentanoic acid (AMKP). The HIL synthesis was catalyzed stereoselectively by an α-ketoglutaric acid-dependent dioxygenase and to be useful for efficient production of a naturally occurring HIL isomer, (2S,3R,4S)-HIL. The (2S,3R,4S)-HIL was oxidized to (2S,3R)-AMKP by a NAD(+)-dependent dehydrogenase. The metabolic pathway functions as an effective bypass pathway that compensates for the incomplete tricarboxylic acid (TCA) cycle in Bacillus species and also explains how AMKP, a vitamin B(12) antimetabolite with antibiotic activity, is synthesized. These novel findings pave a new way for the commercial production of HIL and also for AMKP. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Springer-Verlag | en |
dc.rights | The final publication is available at www.springerlink.com | en |
dc.subject | L-Isoleucine dioxygenase | en |
dc.subject | 4-Hydroxyisoleucine | en |
dc.subject | NAD(+)-dependent dehydrogenase | en |
dc.subject | 2-Amino-3-methyl-4-ketopentanoic acid | en |
dc.subject | Bacillus thuringiensis | en |
dc.subject | TCA cycle | en |
dc.title | A novel L-isoleucine metabolism in Bacillus thuringiensis generating (2S,3R,4S)-4-hydroxyisoleucine, a potential insulinotropic and anti-obesity amino acid. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA11616978 | - |
dc.identifier.jtitle | Applied microbiology and biotechnology | en |
dc.identifier.volume | 89 | - |
dc.identifier.issue | 6 | - |
dc.identifier.spage | 1929 | - |
dc.identifier.epage | 1938 | - |
dc.relation.doi | 10.1007/s00253-010-2983-7 | - |
dc.textversion | publisher | - |
dc.identifier.pmid | 21069315 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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