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j.virol.2011.02.008.pdf | 16.22 MB | Adobe PDF | 見る/開く |
タイトル: | Viral cell-to-cell movement requires formation of cortical punctate structures containing Red clover necrotic mosaic virus movement protein. |
著者: | Kaido, Masanori Funatsu, Naoko Tsuno, Yasuko Mise, Kazuyuki https://orcid.org/0000-0003-4348-6023 (unconfirmed) Okuno, Tetsuro |
著者名の別形: | 海道, 真典 |
キーワード: | Cell-to-cell movement Endoplasmic reticulum Replicase complex Positive-strand RNA virus Dianthovirus |
発行日: | 10-May-2011 |
出版者: | Elsevier Inc. |
誌名: | Virology |
巻: | 413 |
号: | 2 |
開始ページ: | 205 |
終了ページ: | 215 |
抄録: | Movement protein (MP) of Red clover necrotic mosaic virus (RCNMV) forms punctate structures on the cortical endoplasmic reticulum (ER) of Nicotiana benthamiana cells, which are associated with viral RNA1 replication (Kaido et al., Virology 395, 232-242. 2009). We investigated the significance of ER-targeting by MP during virus movement from cell to cell, by analyzing the function of a series of MPs with varying length deletions at their C-terminus, either fused or not fused with green fluorescent protein (GFP). The C-terminal 70 amino acids were crucial to ER-localization of MP-GFP and cell-to-cell movement of the recombinant virus encoding it. However, C-terminal deletion did not affect MP functions, such as increasing the size exclusion limit of plasmodesmata, single-stranded RNA binding in vitro, and MP interacting in vivo. We discuss the possible role of this MP region in virus movement from cell to cell. |
著作権等: | © 2011 Elsevier Inc. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/139997 |
DOI(出版社版): | 10.1016/j.virol.2011.02.008 |
PubMed ID: | 21377183 |
出現コレクション: | 学術雑誌掲載論文等 |
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