Access count of this item: 113

Files in This Item:
File Description SizeFormat 
j.virol.2011.02.008.pdf16.22 MBAdobe PDFView/Open
Title: Viral cell-to-cell movement requires formation of cortical punctate structures containing Red clover necrotic mosaic virus movement protein.
Authors: Kaido, Masanori  kyouindb  KAKEN_id
Funatsu, Naoko
Tsuno, Yasuko
Mise, Kazuyuki  kyouindb  KAKEN_id
Okuno, Tetsuro  KAKEN_id
Author's alias: 海道, 真典
Keywords: Cell-to-cell movement
Endoplasmic reticulum
Replicase complex
Positive-strand RNA virus
Dianthovirus
Issue Date: 10-May-2011
Publisher: Elsevier Inc.
Journal title: Virology
Volume: 413
Issue: 2
Start page: 205
End page: 215
Abstract: Movement protein (MP) of Red clover necrotic mosaic virus (RCNMV) forms punctate structures on the cortical endoplasmic reticulum (ER) of Nicotiana benthamiana cells, which are associated with viral RNA1 replication (Kaido et al., Virology 395, 232-242. 2009). We investigated the significance of ER-targeting by MP during virus movement from cell to cell, by analyzing the function of a series of MPs with varying length deletions at their C-terminus, either fused or not fused with green fluorescent protein (GFP). The C-terminal 70 amino acids were crucial to ER-localization of MP-GFP and cell-to-cell movement of the recombinant virus encoding it. However, C-terminal deletion did not affect MP functions, such as increasing the size exclusion limit of plasmodesmata, single-stranded RNA binding in vitro, and MP interacting in vivo. We discuss the possible role of this MP region in virus movement from cell to cell.
Rights: © 2011 Elsevier Inc.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/139997
DOI(Published Version): 10.1016/j.virol.2011.02.008
PubMed ID: 21377183
Appears in Collections:Journal Articles

Show full item record

Export to RefWorks


Export Format: 


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.