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j.jbiotec.2011.03.024.pdf | 1.31 MB | Adobe PDF | 見る/開く |
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dc.contributor.author | Aihara, Yuki | en |
dc.contributor.author | Fujiwara, Naoko | en |
dc.contributor.author | Yamazaki, Tomohiro | en |
dc.contributor.author | Kambe, Taiho | en |
dc.contributor.author | Nagao, Masaya | en |
dc.contributor.author | Hirose, Yutaka | en |
dc.contributor.author | Masuda, Seiji | en |
dc.contributor.alternative | 増田, 誠司 | ja |
dc.date.accessioned | 2011-06-09T02:19:41Z | - |
dc.date.available | 2011-06-09T02:19:41Z | - |
dc.date.issued | 2011-05-20 | - |
dc.identifier.issn | 0168-1656 | - |
dc.identifier.uri | http://hdl.handle.net/2433/141844 | - |
dc.description.abstract | Recent research into mRNA maturation processes in the nucleus has identified a number of proteins involved in mRNA transcription, capping, splicing, end processing and export. Among them, the Tap-p15 heterodimer acts as an mRNA export receptor. Tap-p15 is recruited onto fully processed mRNA in the nucleus, which is ready for export to the cytoplasm, through associating with Aly or SR proteins on mRNA, or by directly associating with a constitutive transport element (CTE), an RNA element derived from type D retroviruses. mRNA containing a CTE is exported to the cytoplasm by directly associating with Tap-p15, even in the absence of Tap-recruiting proteins such as Aly or SR proteins on the mRNA. Here, we showed that the use of a CTE enhanced the expression of recombinant protein in human cell lines. The co-expression of reporter proteins and Tap-p15 also enhanced recombinant protein expression. Moreover, the use of a CTE and Tap-p15 synergistically further enhanced the recombinant protein expression. In addition to Tap-p15, several Tap-p15-recruiting proteins, including Aly and SR proteins, enhanced recombinant protein expression, albeit independently of the CTE. The incorporation of a CTE and Tap-p15-recruiting proteins into protein expression system is useful to increase recombinant protein yield in human cells. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | en |
dc.rights | © 2011 Elsevier B.V. | en |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.subject | Constitutive transport element | en |
dc.subject | mRNA export | en |
dc.subject | Tap-p15 | en |
dc.subject | mRNA processing factor | en |
dc.title | Enhancing recombinant protein production in human cell lines with a constitutive transport element and mRNA export proteins. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA10458361 | - |
dc.identifier.jtitle | Journal of biotechnology | en |
dc.identifier.volume | 153 | - |
dc.identifier.issue | 04-3 | - |
dc.identifier.spage | 86 | - |
dc.identifier.epage | 91 | - |
dc.relation.doi | 10.1016/j.jbiotec.2011.03.024 | - |
dc.textversion | author | - |
dc.identifier.pmid | 21473891 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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