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nature10106.pdf | 22.32 MB | Adobe PDF | 見る/開く |
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dc.contributor.author | Maekawa, Momoko | en |
dc.contributor.author | Yamaguchi, Kei | en |
dc.contributor.author | Nakamura, Tomonori | en |
dc.contributor.author | Shibukawa, Ran | en |
dc.contributor.author | Kodanaka, Ikumi | en |
dc.contributor.author | Ichisaka, Tomoko | en |
dc.contributor.author | Kawamura, Yoshifumi | en |
dc.contributor.author | Mochizuki, Hiromi | en |
dc.contributor.author | Goshima, Naoki | en |
dc.contributor.author | Yamanaka, Shinya | en |
dc.contributor.alternative | 前川, 桃子 | ja |
dc.contributor.alternative | 山中, 伸弥 | ja |
dc.date.accessioned | 2011-06-22T07:25:05Z | - |
dc.date.available | 2011-06-22T07:25:05Z | - |
dc.date.issued | 2011-06-09 | - |
dc.identifier.citation | Maekawa M, Yamaguchi K, Nakamura T, Shibukawa R, Kodanaka I, Ichisaka T, Kawamura Y, Mochizuki H, Goshima N, Yamanaka S. Direct reprogramming of somatic cells is promoted by maternal transcription factor Glis1. Nature. 2011 Jun 8;474(7350):225-9. doi: 10.1038/nature10106. | - |
dc.identifier.issn | 0028-0836 | - |
dc.identifier.uri | http://hdl.handle.net/2433/141930 | - |
dc.description | 転写因子Glis1により安全なiPS細胞の高効率作製に成功. 京都大学プレスリリース. 2011-06-09. | ja |
dc.description.abstract | Induced pluripotent stem cells (iPSCs) are generated from somatic cells by the transgenic expression of three transcription factors collectively called OSK: Oct3/4 (also called Pou5f1), Sox2 and Klf4. However, the conversion to iPSCs is inefficient. The proto-oncogene Myc enhances the efficiency of iPSC generation by OSK but it also increases the tumorigenicity of the resulting iPSCs. Here we show that the Gli-like transcription factor Glis1 (Glis family zinc finger 1) markedly enhances the generation of iPSCs from both mouse and human fibroblasts when it is expressed together with OSK. Mouse iPSCs generated using this combination of transcription factors can form germline-competent chimaeras. Glis1 is enriched in unfertilized oocytes and in embryos at the one-cell stage. DNA microarray analyses show that Glis1 promotes multiple pro-reprogramming pathways, including Myc, Nanog, Lin28, Wnt, Essrb and the mesenchymal-epithelial transition. These results therefore show that Glis1 effectively promotes the direct reprogramming of somatic cells during iPSC generation. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Nature Publishing Group | en |
dc.rights | © 2011 Nature Publishing Group | en |
dc.rights | 許諾条件により本文は2011-12-09に公開. | ja |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.title | Direct reprogramming of somatic cells is promoted by maternal transcription factor Glis1. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA00752384 | - |
dc.identifier.jtitle | Nature | en |
dc.identifier.volume | 474 | - |
dc.identifier.issue | 7350 | - |
dc.identifier.spage | 225 | - |
dc.identifier.epage | 229 | - |
dc.relation.doi | 10.1038/nature10106 | - |
dc.textversion | author | - |
dc.startdate.bitstreamsavailable | 2011-12-09 | - |
dc.identifier.pmid | 21654807 | - |
dc.relation.url | http://www.nature.com/nature/journal/v474/n7350/pdf/nature10106.pdf | - |
dc.relation.url | https://www.kyoto-u.ac.jp/static/ja/news_data/h/h1/news6/2011/110609_1.htm | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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