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タイトル: | Multiplex PCR for the concurrent detection and differentiation of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium |
著者: | Pui, Chai Fung Wong, Woan Chwen Chai, Lay Ching Lee, Hai Yen Noorlis, Ahmad Zainazor, Tuan Chilek Tuan Tang, John Yew Huat Ghazali, Farinazleen Mohamad Cheah, Yoke Kqueen Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son |
著者名の別形: | 西渕, 光昭 |
キーワード: | optimization multiplex PCR Salmonella spp. Salmonella Typhi Salmonella Typhimurium |
発行日: | 2011 |
出版者: | Japanese Society of Tropical Medicine |
誌名: | Tropical Medicine and Health |
巻: | 39 |
号: | 1 |
開始ページ: | 9 |
終了ページ: | 15 |
抄録: | Salmonellosis outbreaks involving typhoid fever and human gastroenteritis are important diseases in tropical countries where hygienic conditions are often not maintained. A rapid and sensitive method to detect Salmonella spp., Salmonella Typhi and Salmonella Typhimurium is needed to improve control and surveillance of typhoid fever and Salmonella gastroenteritis. Our objective was the concurrent detection and differentiation of these food-borne pathogens using a multiplex PCR. We therefore designed and optimized a multiplex PCR using three specific PCR primer pairs for the simultaneous detection of these pathogens. The concentration of each of the primer pairs, magnesium chloride concentration, and primer annealing temperature were optimized before verification of the specificity of the primer pairs. The target genes produced amplicons at 429 bp, 300 bp and 620 bp which were shown to be 100% specific to each target bacterium, Salmonella spp., Salmonella Typhi and Salmonella Typhimurium, respectively. |
著作権等: | (c) 2011 by The Japanese Society of Tropical Medicine |
URI: | http://hdl.handle.net/2433/142312 |
DOI(出版社版): | 10.2149/tmh.2010-20 |
PubMed ID: | 22028607 |
出現コレクション: | 学術雑誌掲載論文等 |
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