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j.joca.2011.04.007.pdf | 16.3 MB | Adobe PDF | 見る/開く |
タイトル: | Cartilaginous repair of full-thickness articular cartilage defects is induced by the intermittent activation of PTH/PTHrP signaling. |
著者: | Kudo, Satoshi Mizuta, Hiroshi Takagi, Katsumasa Hiraki, Yuji ![]() |
著者名の別形: | 開, 祐司 |
キーワード: | PTH/PTHrP signaling Chondrogenesis Tissue repair Articular cartilage Osteochondral defects |
発行日: | Jul-2011 |
出版者: | Elsevier Ltd. |
誌名: | Osteoarthritis and cartilage |
巻: | 19 |
号: | 7 |
開始ページ: | 886 |
終了ページ: | 894 |
抄録: | Objective:We studied the effects of the transient activation of parathyroid hormone (PTH)/PTH-related peptide (PTHrP) signaling during the repair of 5-mm-diameter full-thickness defects of articular cartilage in the rabbit. Materials and methods:Cylindrical full-thickness articular cartilage defects of 5 mm in diameter were artificially created in the femoral trochlea of male adolescent Japanese white rabbits using a hand-drill. Recombinant human PTH(1-84) was then administered into the joint cavity continuously or intermittently for 2 weeks post-injury. The reparative tissues were histologically examined at 2, 4, and 8 weeks, and were also immunohistochemically examined for type II collagen. Double immunostaining analysis was also performed for the PTH/PTHrP receptor and proliferating cell nuclear antigen (PCNA) in the regenerating tissues. Results:No evidence of cartilage formation was evident throughout the period of the experiments in injured animals administered saline alone. In contrast, cartilage formation occurred at 4 weeks in both the continuous and intermittent PTH-treated defects. At 8 weeks post-injury, for the intermittently treated defects, the regenerated cartilage successfully resurfaced the defects and the original bone-articular cartilage junction was recovered. In contrast, the defects were covered with fibrous or fibrocartilaginous tissues in the continuously administered group. PCNA and PTH/PTHrP receptor-double positive mesenchymal cells were significantly increased in both the continuous and intermittent PTH-treated defects at 2 weeks post-injury. Conclusions:The present results suggest that the transient activation and release from PTH/PTHrP signaling during the early stages of the cartilage repair process facilitates the induction of regenerative chondrogenesis in full-thickness articular cartilage defects. |
著作権等: | Copyright © 2011 Osteoarthritis Research Society International Published by Elsevier Ltd. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/143679 |
DOI(出版社版): | 10.1016/j.joca.2011.04.007 |
PubMed ID: | 21571083 |
出現コレクション: | 学術雑誌掲載論文等 |
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