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Title: Inhibition of surgical trauma-enhanced peritoneal dissemination of tumor cells by human catalase derivatives in mice.
Authors: Nishizaki, Chika
Nishikawa, Makiya  kyouindb  KAKEN_id
Yata, Tomoya
Yamada, Toshiyuki
Takahashi, Yuki  kyouindb  KAKEN_id  orcid (unconfirmed)
Oku, Masahide  kyouindb  KAKEN_id  orcid (unconfirmed)
Yurimoto, Hiroya  kyouindb  KAKEN_id  orcid (unconfirmed)
Sakai, Yasuyoshi  kyouindb  KAKEN_id
Nakanishi, Kenji
Takakura, Yoshinobu  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 西川, 元也
Keywords: Recombinant human catalase
Surgical trauma
Tumor metastasis
Reactive oxygen species
Free radicals
Issue Date: 1-Aug-2011
Publisher: Elsevier BV
Journal title: Free radical biology & medicine
Volume: 51
Issue: 3
Start page: 773
End page: 779
Abstract: Surgical trauma, which is inevitably associated with the surgical removal of cancer, has been reported to accelerate tumor metastasis. The close association of reactive oxygen species with the trauma and tumor metastasis supports the possibility of using antioxidants for the inhibition of metastasis. To inhibit surgical trauma-enhanced peritoneal dissemination, human catalase (hCAT) derivatives, i.e., hCAT-nona-arginine peptide (hCAT-R9) and hCAT-albumin-binding peptide (hCAT-ABP), were designed to increase the retention time of the antioxidant enzyme in the abdominal cavity after intraperitoneal administration. Both (125)I-labeled derivatives showed significantly prolonged retention in the cavity compared to (125)I-hCAT. Cauterization of the cecum of mice with a hot iron, an experimental model of surgical trauma, induced abdominal adhesions. In addition, cauterization followed by colon26 tumor cell inoculation increased lipid peroxidation in the cecum and mRNA expression of molecules associated with tissue repair/adhesion and inflammation in the peritoneum. hCAT derivatives significantly suppressed the increased mRNA expression. The cauterization also increased the number of tumor cells in the abdominal organs, and the number was significantly reduced by hCAT-R9 or hCAT-ABP. These results indicate that hCAT-R9 and hCAT-ABP, both of which have a long retention time in the peritoneal cavity, can be effective at inhibiting surgery-induced peritoneal metastasis.
Rights: © 2011 Elsevier Inc.
This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1016/j.freeradbiomed.2011.05.025
PubMed ID: 21664457
Appears in Collections:Journal Articles

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