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Title: A Novel Serum-Free Monolayer Culture for Orderly Hematopoietic Differentiation of Human Pluripotent Cells via Mesodermal Progenitors.
Authors: Niwa, Akira  kyouindb  KAKEN_id
Heike, Toshio  kyouindb  KAKEN_id
Umeda, Katsutsugu  kyouindb  KAKEN_id
Oshima, Koichi
Kato, Itaru  kyouindb  KAKEN_id
Sakai, Hiromi
Suemori, Hirofumi  kyouindb  KAKEN_id  orcid (unconfirmed)
Nakahata, Tatsutoshi  kyouindb  KAKEN_id
Saito, Megumu K
Author's alias: 斎藤, 潤
Issue Date: Jul-2011
Publisher: Public Library of Science
Journal title: PloS one
Volume: 6
Issue: 7
Thesis number: e22261
Abstract: Elucidating the in vitro differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells is important for understanding both normal and pathological hematopoietic development in vivo. For this purpose, a robust and simple hematopoietic differentiation system that can faithfully trace in vivo hematopoiesis is necessary. In this study, we established a novel serum-free monolayer culture that can trace the in vivo hematopoietic pathway from ES/iPS cells to functional definitive blood cells via mesodermal progenitors. Stepwise tuning of exogenous cytokine cocktails induced the hematopoietic mesodermal progenitors via primitive streak cells. These progenitors were then differentiated into various cell lineages depending on the hematopoietic cytokines present. Moreover, single cell deposition assay revealed that common bipotential hemoangiogenic progenitors were induced in our culture. Our system provides a new, robust, and simple method for investigating the mechanisms of mesodermal and hematopoietic differentiation.
Rights: © 2011 Niwa et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI(Published Version): 10.1371/journal.pone.0022261
PubMed ID: 21818303
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