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ファイル | 記述 | サイズ | フォーマット | |
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j.molcel.2011.07.026.pdf | 6.96 MB | Adobe PDF | 見る/開く |
タイトル: | NBS1 Recruits RAD18 via a RAD6-like Domain and Regulates Pol η-Dependent Translesion DNA Synthesis. |
著者: | Yanagihara, Hiromi Kobayashi, Junya Tateishi, Satoshi Kato, Akihiro Matsuura, Shinya Tauchi, Hiroshi Yamada, Kouichi Takezawa, Jun Sugasawa, Kaoru Masutani, Chikahide Hanaoka, Fumio Weemaes, Corry M Mori, Toshio Zou, Lee Komatsu, Kenshi |
著者名の別形: | 柳原, 啓見 小松, 賢志 |
発行日: | 2-Sep-2011 |
出版者: | Elsevier Ltd. |
引用: | Yanagihara H, Kobayashi J, Tateishi S, Kato A, Matsuura S, Tauchi H, Yamada K, Takezawa J, Sugasawa K, Masutani C, Hanaoka F, Weemaes CM, Mori T, Zou L, Komatsu K. NBS1 Recruits RAD18 via a RAD6-like Domain and Regulates Pol η-Dependent Translesion DNA Synthesis. Mol Cell. 2011 Sep 2;43(5):788-97. |
誌名: | Molecular cell |
巻: | 43 |
号: | 5 |
開始ページ: | 788 |
終了ページ: | 797 |
抄録: | Translesion DNA synthesis, a process orchestrated by monoubiquitinated PCNA, is critical for DNA damage tolerance. While the ubiquitin-conjugating enzyme RAD6 and ubiquitin ligase RAD18 are known to monoubiquitinate PCNA, how they are regulated by DNA damage is not fully understood. We show that NBS1 (mutated in Nijmegen breakage syndrome) binds to RAD18 after UV irradiation and mediates the recruitment of RAD18 to sites of DNA damage. Disruption of NBS1 abolished RAD18-dependent PCNA ubiquitination and Polη focus formation, leading to elevated UV sensitivity and mutation. Unexpectedly, the RAD18-interacting domain of NBS1, which was mapped to its C terminus, shares structural and functional similarity with the RAD18-interacting domain of RAD6. These domains of NBS1 and RAD6 allow the two proteins to interact with RAD18 homodimers simultaneously and are crucial for Polη-dependent UV tolerance. Thus, in addition to chromosomal break repair, NBS1 plays a key role in translesion DNA synthesis. |
記述: | 放射線の修復蛋白NBS1放によるRAD18を介した損傷乗り越えDNA合成の開始. 京都大学プレスリリース. 2011-09-02. |
著作権等: | © 2011 Elsevier Inc. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/145963 |
DOI(出版社版): | 10.1016/j.molcel.2011.07.026 |
PubMed ID: | 21884979 |
関連リンク: | http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WSR-53P6C29-C-1&_cdi=7053&_user=119230&_pii=S1097276511005910&_origin=gateway&_coverDate=09%2F02%2F2011&_sk=999569994&view=c&wchp=dGLzVlb-zSkWl&md5=3038c913933c6eac2c2987f4de4385e1&ie=/sdarticle.pdf http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2011/110902_1.htm |
出現コレクション: | 学術雑誌掲載論文等 |
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