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j.biomaterials.2011.05.076.pdf9.2 MBAdobe PDF見る/開く
タイトル: Polyamidoamine dendrimer-conjugated quantum dots for efficient labeling of primary cultured mesenchymal stem cells.
著者: Higuchi, Yuriko  kyouindb  KAKEN_id
Wu, Can
Chang, Kai-Ling
Irie, Kei
Kawakami, Shigeru
Yamashita, Fumiyoshi  kyouindb  KAKEN_id
Hashida, Mitsuru  kyouindb  KAKEN_id
著者名の別形: 樋口, ゆり子
キーワード: Quantum dot
Stem cell
In vivo imaging
PAMAM dendrimer
Endosomal escape
Buffering effect
発行日: Oct-2011
出版者: Elsevier Ltd.
誌名: Biomaterials
巻: 32
号: 28
開始ページ: 6676
終了ページ: 6682
抄録: Monitoring of cells in vivo after transplantation could supply important information for determining the efficacy of stem cell therapy. The use of quantum dots (QDs) has several advantages for in vivo imaging, such as remarkable resistance to photo bleaching, high fluorescence efficiency, and size-tunable emission. After they are taken up by cells via endocytosis, QDs lose their fluorescence intensity in endosomes/lysosomes at low pH because the intensity cannot survive under acidic conditions. Moreover, the amount of QD uptake by mesenchymal stem cells (MSCs) is extremely small. Therefore, for effective labeling of MSCs and long observation of MSCs labeled by QDs in vivo, it is essential both to increase cellular uptake of QDs and to promote endosomal escape into the cytosol. The polyamidoamine (PAMAM) dendrimer had plenty of cationic charge, which promoted cellular uptake though electrostatic interactions, and a "buffering capacity, " which enhanced endosomal escape into the cytosol. In this study, QDs were modified with PAMAM dendrimer for the efficient labeling of MSCs by QDs. The uptake efficiency and cytosolic distribution of QDs in primary cultured MSCs were increased by the modification of the PAMAM dendrimer. The fluorescence intensity in MSCs labeled by PAMAM dendrimer-conjugated QDs lasted for a longer time in harvested culture plates or in cell-transplanted mice than that in MSCs labeled by non-conjugated QDs.
著作権等: © 2011 Elsevier Ltd.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/147161
DOI(出版社版): 10.1016/j.biomaterials.2011.05.076
PubMed ID: 21700331
出現コレクション:学術雑誌掲載論文等

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