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Title: Involvement of activated transcriptional process in efficient gene transfection using unmodified and mannose-modified bubble lipoplexes with ultrasound exposure.
Authors: Un, Keita
Kawakami, Shigeru
Higuchi, Yuriko  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-9869-1019 (unconfirmed)
Suzuki, Ryo  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-3503-8696 (unconfirmed)
Maruyama, Kazuo
Yamashita, Fumiyoshi  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-3503-8696 (unconfirmed)
Hashida, Mitsuru  kyouindb  KAKEN_id
Author's alias: 運, 敬太
川上, 茂
Keywords: Gene transfection
Bubble lipoplex
Ultrasound
Transcription factor
Inflammatory response
Issue Date: 20-Dec-2011
Publisher: Elsevier BV
Journal title: Journal of controlled release
Volume: 156
Issue: 3
Start page: 355
End page: 363
Abstract: Recently, our group developed ultrasound (US)-responsive and mannose-modified gene carriers (Man-PEG(2000) bubble lipoplexes), and successfully obtained a high level of gene expression in mannose receptor-expressing cells following gene transfection using Man-PEG(2000) bubble lipoplexes and US exposure. We also reported that large amounts of plasmid DNA (pDNA) were transferred into the cytoplasm of the targeted cells in the gene transfection using this method. In the present study, we investigated the involvement of transcriptional processes on enhanced gene expression obtained by unmodified and Man-PEG(2000) bubble lipoplexes with US exposure. The transcriptional process related to activator protein-1 (AP-1) and nuclear factor-κB (NFκB) was activated by US exposure, and was founded to be involved in enhanced gene expression obtained by gene transfection using unmodified and Man-PEG(2000) bubble lipoplexes with US exposure. On the other hand, activation of AP-1 and NFκB pathways followed by US exposure was hardly involved in the inflammatory responses in the gene transfection using this method. These findings suggest that activation of AP-1 and NFκB followed by US exposure is involved in the enhanced gene expression using unmodified and Man-PEG(2000) bubble lipoplexes with US exposure, and the selection of pDNAs activated by US exposure is important in this gene transfection method.
Rights: © 2011 Elsevier B.V.
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
This is not the published version. Please cite only the published version.
URI: http://hdl.handle.net/2433/151860
DOI(Published Version): 10.1016/j.jconrel.2011.06.040
PubMed ID: 21756951
Appears in Collections:Journal Articles

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