ダウンロード数: 326
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
g11-088.pdf | 1.31 MB | Adobe PDF | 見る/開く |
完全メタデータレコード
DCフィールド | 値 | 言語 |
---|---|---|
dc.contributor.author | Koga, Akihiko | en |
dc.contributor.alternative | 古賀, 章彦 | ja |
dc.date.accessioned | 2012-04-04T07:14:04Z | - |
dc.date.available | 2012-04-04T07:14:04Z | - |
dc.date.issued | 2012-02 | - |
dc.identifier.issn | 0831-2796 | - |
dc.identifier.uri | http://hdl.handle.net/2433/154764 | - |
dc.description.abstract | It is widely accepted in a conceptual framework that repetitive sequences, especially those with high sequence homogeneity among copies, tend to be under-represented in whole-genome shotgun sequence databases, because of the difficulty of assembling sequence reads into contigs. Although this is easily inferred, there is no quantitative illustration of this phenomenon. An example using a currently used database is expected to contribute to the intuitive understanding of how serious the under-representation is. The present study provides the first quantitative example (in the case of 16 copies of virtually identical, 4.7-kb sequences in a genome of 7 × 10 (8) bp) by comparing the results of BLAST searches of a sequence database (contig N50; 9.8 kb) with those of Southern blot analysis of genomic DNA. This has revealed that the internal regions of the repetitive sequences are under-represented to a striking extent. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Canadian Science Publishing | en |
dc.rights | © Copyright 2012 – Canadian Science Publishing. | en |
dc.rights | この論文は著者最終稿です。内容が印刷版と異なることがありますので、引用の際には出版社版をご確認ご利用ください。This is the Accepted Author Manuscript. Please cite only the published version. | ja |
dc.subject | contig | en |
dc.subject | database | en |
dc.subject | repetitive sequences | en |
dc.subject | under-representation | en |
dc.subject | transposon | en |
dc.title | Under-representation of repetitive sequences in whole-genome shotgun sequence databases: an illustration using a recently acquired transposable element. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Genome / National Research Council Canada | en |
dc.identifier.volume | 55 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 172 | - |
dc.identifier.epage | 175 | - |
dc.relation.doi | 10.1139/g11-088 | - |
dc.textversion | author | - |
dc.identifier.pmid | 22321171 | - |
dcterms.accessRights | open access | - |
dc.identifier.jtitle-alternative | Génome / Conseil national de recherches Canada | fr |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。