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| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| cbl02701_001.pdf | 2.07 MB | Adobe PDF | 見る/開く |
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| DCフィールド | 値 | 言語 |
|---|---|---|
| dc.contributor.author | MARUYAMA, Keizo | en |
| dc.contributor.author | OKUDA, Mitsuro | en |
| dc.contributor.alternative | 丸山, 圭藏 | ja |
| dc.contributor.alternative | 奥田, 光郎 | ja |
| dc.contributor.transcription | マルヤマ, ケイゾウ | ja-Kana |
| dc.contributor.transcription | オクダ, ミツロウ | ja-Kana |
| dc.date.accessioned | 2012-06-01T04:34:41Z | - |
| dc.date.available | 2012-06-01T04:34:41Z | - |
| dc.date.issued | 1985-08-23 | - |
| dc.identifier.issn | 0452-9987 | - |
| dc.identifier.uri | http://hdl.handle.net/2433/156083 | - |
| dc.description.abstract | Conditions of freezing and thawing of Vicia root cells for scanning electron microscopy (SEM) were investigated. The best result was obtained when the roots were frozen directly in liquid Freon without pretreatment with a cryoprotectant DMSO (dimethyl sulfoxide). The damage caused by the absence of DMSO, if present, was less than that caused by DMSO pretreatment. The composition of the thawing solution was important. A high concentration (40%) of DMSO in the thawing solution probably served to lessen the freeze-thawing damage. | en |
| dc.format.mimetype | application/pdf | - |
| dc.language.iso | eng | - |
| dc.publisher | 京都大学教養部生物学教室 | ja |
| dc.publisher.alternative | Biological Laboratory, Kyoto University | en |
| dc.subject | freeze-fracture | en |
| dc.subject | root meristem | en |
| dc.subject | Vicia faba | en |
| dc.subject | scanning electron microscopy | en |
| dc.subject.ndc | 460 | - |
| dc.title | Freeze-Fracture and Thaw-Fixation of Vicia Root Cells for Scanning Electron Microscopy (SEM) | en |
| dc.type | departmental bulletin paper | - |
| dc.type.niitype | Departmental Bulletin Paper | - |
| dc.identifier.ncid | AA00616874 | - |
| dc.identifier.jtitle | Contributions from the Biological Laboratory, Kyoto University | en |
| dc.identifier.volume | 27 | - |
| dc.identifier.issue | 1 | - |
| dc.identifier.spage | 1 | - |
| dc.identifier.epage | 8 | - |
| dc.textversion | publisher | - |
| dc.sortkey | 02 | - |
| dc.address | Biological Laboratory, Yoshida College, Kyoto University | en |
| dc.address | Biological Laboratory, Yoshida College, Kyoto University | en |
| dcterms.accessRights | open access | - |
| dc.identifier.pissn | 0452-9987 | - |
| 出現コレクション: | Vol.27 No.1 | |
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