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Title: Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis
Authors: Makyio, Hisayoshi
Ohgi, Minako
Takei, Tomomi
Takahashi, Senye
Takatsu, Hiroyuki
Katoh, Yohei  kyouindb  KAKEN_id
Hanai, Ayako
Ueda, Tomoko
Kanaho, Yasunori
Xie, Yong
Shin, Hye-Won  kyouindb  KAKEN_id
Kamikubo, Hironari
Kataoka, Mikio
Kawasaki, Masato
Kato, Ryuichi
Wakatsuki, Soichi
Nakayama, Kazuhisa  kyouindb  KAKEN_id
Author's alias: 中山, 和久
Keywords: Arf6
cytokinesis
Flemming body
MKLP1
X-ray crystallography
Issue Date: 30-May-2012
Publisher: Nature Publishing Group
Citation: Makyio Hisayoshi, Ohgi Minako, Takei Tomomi, Takahashi Senye, Takatsu Hiroyuki, Katoh Yohei, Hanai Ayako, Ueda Tomoko, Kanaho Yasunori, Xie Yong, Shin Hye-Won, Kamikubo Hironari, Kataoka Mikio, Kawasaki Masato, Kato Ryuichi, Wakatsuki Soichi, Nakayama Kazuhisa. Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO Journal, Vol. 31, No. 11, pp. 2590-2603 (May 30, 2012). doi: 10.1038/emboj.2012.89
Journal title: The EMBO journal
Volume: 31
Issue: 11
Start page: 2590
End page: 2603
Abstract: A small GTPase, Arf6, is involved in cytokinesis by localizing to the Flemming body (the midbody). However, it remains unknown how Arf6 contributes to cytokinesis. Here, we demonstrate that Arf6 directly interacts with mitotic kinesin-like protein 1 (MKLP1), a Flemming body-localizing protein essential for cytokinesis. The crystal structure of the Arf6-MKLP1 complex reveals that MKLP1 forms a homodimer flanked by two Arf6 molecules, forming a 2:2 heterotetramer containing an extended β-sheet composed of 22 β-strands that spans the entire heterotetramer, suitable for interaction with a concave membrane surface at the cleavage furrow. We show that, during cytokinesis, Arf6 is first accumulated around the cleavage furrow and, prior to abscission, recruited onto the Flemming body via interaction with MKLP1. We also show by structure-based mutagenesis and siRNA-mediated knockdowns that the complex formation is required for completion of cytokinesis. A model based on these results suggests that the Arf6-MKLP1 complex plays a crucial role in cytokinesis by connecting the microtubule bundle and membranes at the cleavage plane.
Description: 正常な細胞分裂に不可欠なタンパク質の機能と構造を解明. 京都大学プレスリリース. 2012-05-31.
Rights: © 2012 European Molecular Biology Organization. 許諾条件により本文は2012-11-30に公開.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URL: http://www.nature.com/emboj/journal/v31/n11/full/emboj201289a.html
URI: http://hdl.handle.net/2433/156131
DOI(Published Version): 10.1038/emboj.2012.89
PubMed ID: 22522702
Related Link: http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2012/120531_1.htm
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