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j.bbrc.2012.04.045.pdf | 747.37 kB | Adobe PDF | 見る/開く |
タイトル: | Conversion of 3-oxo steroids into ecdysteroids triggers molting and expression of 20E-inducible genes in Drosophila melanogaster. |
著者: | Ono, Hajime ![]() ![]() ![]() Morita, Sayo Asakura, Ichiyoh Nishida, Ritsuo ![]() |
著者名の別形: | 小野, 肇 |
キーワード: | Ecdysteroidogenesis Ecdysone 3-Oxo-steroid Molting Drosophila melanogaster |
発行日: | 11-May-2012 |
出版者: | Elsevier Inc. |
誌名: | Biochemical and biophysical research communications |
巻: | 421 |
号: | 3 |
開始ページ: | 561 |
終了ページ: | 566 |
抄録: | Ecdysteroids, steroid hormones in insects, coordinate major developmental transitions. During postembryonic development, ecdysone is biosynthesized from dietary cholesterol in the prothoracic gland (PG). Despite extensive studies, the initial conversion process, the so-called "Black Box", has not been characterized. A cytochrome P450 enzyme, Spookier (Spok), is speculated as a rate limiting enzyme in the Black Box during larval-pupal transitions in Drosophila melanogaster. RNAi mediated knockdown of spok expression in the PG results in arrest of molting. Because the developmental arrest can be rescued by application of an appropriate intermediate, we examined potential activities of candidate intermediates in the RNAi-treated larvae. We found that two 3-oxo steroids, cholesta-4, 7-diene-3, 6-dione-14α-ol (Δ(4)-diketol) and 5β [H]cholesta-7-ene-3, 6-dione-14α-ol (diketol), triggered molting of the RNAi-treated larvae. We also detected an enhancement of the amounts of ecdysteroids in the RNAi-treated larvae by feeding the Δ(4)-diketol or diketol, indicating that the dietary 3-oxo steroids were incorporated and converted into ecdysteroids in vivo. Furthermore, 20-hydroxyecdysone inducible genes were induced in the RNAi-treated larvae by feeding the Δ(4)-diketol or diketol. These results indicate that Δ(4)-diketol and diketol are components of the ecdysteroid biosynthetic pathway and lie downstream of a step catalyzed by Spok. |
著作権等: | © 2012 Elsevier Inc. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/156141 |
DOI(出版社版): | 10.1016/j.bbrc.2012.04.045 |
PubMed ID: | 22525676 |
出現コレクション: | 学術雑誌掲載論文等 |
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