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タイトル: | A Mechanistic Analysis of Enzymatic Degradation of Organohalogen Compounds |
著者: | KURIHARA, Tatsuo https://orcid.org/0000-0002-7777-1378 (unconfirmed) |
著者名の別形: | 栗原, 達夫 |
キーワード: | L-2-haloacid dehalogenase DL-2-haloacid dehalogenase fluoroacetate dehalogenase 2-haloacrylate reductase 2-haloacrylate hydratase |
発行日: | Feb-2011 |
出版者: | Japan Society for Bioscience, Biotechnology, and Agrochemistry |
誌名: | Bioscience, Biotechnology, and Biochemistry |
巻: | 75 |
号: | 2 |
開始ページ: | 189 |
終了ページ: | 198 |
抄録: | Enzymes that catalyze the conversion of organohalogen compounds have been attracting a great deal of attention, partly because of their possible applications in environmental technology and the chemical industry. We have studied the mechanisms of enzymatic degradation of various organic halo acids. In the reaction of L-2-haloacid dehalogenase and fluoroacetate dehalogenase, the carboxylate group of the catalytic aspartate residue nucleophilically attacked the α-carbon atom of the substrates to displace the halogen atom. In the reaction catalyzed by DL-2-haloacid dehalogenase, a water molecule directly attacked the substrate to displace the halogen atom. In the course of studies on the metabolism of 2-chloroacrylate, we discovered two new enzymes. 2-Haloacrylate reductase catalyzed the asymmetric reduction of 2-haloacrylate to produce L-2-haloalkanoic acid in an NADPH-dependent manner. 2-Haloacrylate hydratase catalyzed the hydration of 2-haloacrylate to produce pyruvate. The enzyme is unique in that it catalyzes the non-redox reaction in an FADH2-dependent manner. |
著作権等: | (c) 2011 by Japan Society for Bioscience, Biotechnology, and Agrochemistry. |
URI: | http://hdl.handle.net/2433/156503 |
DOI(出版社版): | 10.1271/bbb.100746 |
PubMed ID: | 21307570 |
関連リンク: | https://www.jstage.jst.go.jp/article/bbb/75/2/75_100746/_article |
出現コレクション: | 学術雑誌掲載論文等 |
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