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タイトル: | Isoquinoline alkaloid biosynthesis is regulated by a unique bHLH-type transcription factor in Coptis japonica. |
著者: | Yamada, Yasuyuki Kokabu, Yasuhisa Chaki, Kaori Yoshimoto, Tadashi Ohgaki, Mai Yoshida, Sayumi Kato, Nobuhiko Koyama, Tomotsugu Sato, Fumihiko |
著者名の別形: | 佐藤, 文彦 |
キーワード: | Basic helix–loop–helix Coptis japonica Isoquinoline alkaloid biosynthesis Transcriptional regulation |
発行日: | Jul-2011 |
出版者: | Oxford University Press |
誌名: | Plant & cell physiology |
巻: | 52 |
号: | 7 |
開始ページ: | 1131 |
終了ページ: | 1141 |
抄録: | Specific plant species produce unique isoquinoline alkaloids (IQAs); however, the mechanism of their evolution and the regulation of their biosynthesis are largely unknown. We report here the isolation of a novel basic helix-loop-helix protein, CjbHLH1, from IQA-producing Coptis japonica. A BLAST search indicated that CjbHLH1 homologs were only found in plant species that produce IQAs. Transient RNA interference (RNAi) and overexpression of CjbHLH1 in C. japonica protoplasts revealed the activity of CjbHLH1 in transcription of IQA biosynthetic genes, and little activity in the transcription of genes involved in primary metabolism or the stress response. A chromatin immunoprecipitation experiment using CjbHLH1-specific antibodies revealed the direct interaction of CjbHLH1 with promoter sequences of IQA biosynthetic genes in vivo. We discuss the unique role of CjbHLH1 in IQA biosynthesis. |
著作権等: | © The Author 2011. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/156870 |
DOI(出版社版): | 10.1093/pcp/pcr062 |
PubMed ID: | 21576193 |
出現コレクション: | 学術雑誌掲載論文等 |
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