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Title: Identification and characterization of an oocyte factor required for development of porcine nuclear transfer embryos.
Authors: Miyamoto, Kei
Nagai, Kouhei
Kitamura, Naoya
Nishikawa, Tomoaki
Ikegami, Haruka
Binh, Nguyen T
Tsukamoto, Satoshi
Matsumoto, Mai
Tsukiyama, Tomoyuki
Minami, Naojiro  kyouindb  KAKEN_id
Yamada, Masayasu  kyouindb  KAKEN_id
Ariga, Hiroyoshi
Miyake, Masashi
Kawarasaki, Tatsuo
Matsumoto, Kazuya
Imai, Hiroshi  kyouindb  KAKEN_id
Author's alias: 宮本, 圭
Issue Date: 26-Apr-2011
Publisher: National Academy of Sciences
Journal title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 108
Issue: 17
Start page: 7040
End page: 7045
Abstract: Nuclear reprogramming of differentiated cells can be induced by oocyte factors. Despite numerous attempts, these factors and mechanisms responsible for successful reprogramming remain elusive. Here, we identify one such factor, necessary for the development of nuclear transfer embryos, using porcine oocyte extracts in which some reprogramming events are recapitulated. After incubating somatic nuclei in oocyte extracts from the metaphase II stage, the oocyte proteins that were specifically and abundantly incorporated into the nuclei were identified by mass spectrometry. Among 25 identified proteins, we especially focused on a multifunctional protein, DJ-1. DJ-1 is present at a high concentration in oocytes from the germinal vesicle stage until embryos at the four-cell stage. Inhibition of DJ-1 function compromises the development of nuclear transfer embryos but not that of fertilized embryos. Microarray analysis of nuclear transfer embryos in which DJ-1 function is inhibited shows perturbed expression of P53 pathway components. In addition, embryonic arrest of nuclear transfer embryos injected with anti-DJ-1 antibody is rescued by P53 inhibition. We conclude that DJ-1 is an oocyte factor that is required for development of nuclear transfer embryos. This study presents a means for identifying natural reprogramming factors in mammalian oocytes and a unique insight into the mechanisms underlying reprogramming by nuclear transfer.
Rights: ©2011 by the National Academy of Sciences
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/159448
DOI(Published Version): 10.1073/pnas.1013634108
PubMed ID: 21482765
Appears in Collections:Journal Articles

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