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dc.contributor.author | Hori, Kimiko | en |
dc.contributor.author | Miyamoto, Shin'ichi | en |
dc.contributor.author | Yukawa, Yoshiyuki | en |
dc.contributor.author | Muto, Manabu | en |
dc.contributor.author | Chiba, Tsutomu | en |
dc.contributor.author | Matsuda, Tomonari | en |
dc.contributor.alternative | 武藤, 学 | ja |
dc.date.accessioned | 2012-10-15T02:26:11Z | - |
dc.date.available | 2012-10-15T02:26:11Z | - |
dc.date.issued | 2012-07-13 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/2433/160034 | - |
dc.description.abstract | Acetaldehyde (AA) derived from alcoholic beverages is a confirmed carcinogen for esophageal and head and neck cancers. AA forms various DNA adducts and is thought to play a crucial role in carcinogenesis. Transient DNA adducts are usually repaired, but the stability of AA-derived DNA adducts has not been elucidated. We investigated the stability of N(2)-ethylidene-2'-deoxyguanosine (N(2)-ethylidene-dG), a major AA-derived DNA adduct, in cultured cells. First, to determine the optimal concentration of AA for detecting N(2)-ethylidene-dG in cell culture, a dose-response study was performed using HL60 cells of the human promyelocytic leukemia cell line. An AA concentration ≥ 0.01% (1.8 mM) was required to detect N(2)-ethylidene-dG in vitro. We next examined the stability of N(2)-ethylidene-dG. After a 1 or 2h exposure to 0.01% of AA in a tightly sealed bottle, N(2)-ethylidene-dG content was measured by sensitive liquid chromatography tandem mass spectrometry immediately, 24h, and 48 h after exposure. After the 1h exposure, the mean (± SD) N(2)-ethylidene-dG contents were 12.1 ± 1.28, 8.20 ± 0.64, and 6.70 ± 0.52 adducts per 10(7) bases at each postexposure time. After the 2h exposure, N(2)-ethylidene-dG content increased to 21.4 ± 7.50, 10.5 ± 3.61, and 9.83 ± 3.90 adducts per 10(7) bases at each postexposure time. The half-life of this adduct was calculated as ∼35 h in independent experiments. These results indicate that AA exposure from daily alcohol consumption may cause DNA damage and may increase the risk of alcohol-related carcinogenesis. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier Inc. | en |
dc.rights | © 2012 Elsevier Inc. | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.subject | Alcohol | en |
dc.subject | Acetaldehyde | en |
dc.subject | DNA adduct | en |
dc.subject | N(2)-ethylidene-2′-deoxyguanosine | en |
dc.subject | Esophageal cancer | en |
dc.subject | Carcinogenesis | en |
dc.title | Stability of acetaldehyde-derived DNA adduct in vitro. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA00564395 | - |
dc.identifier.jtitle | Biochemical and biophysical research communications | en |
dc.identifier.volume | 423 | - |
dc.identifier.issue | 4 | - |
dc.identifier.spage | 642 | - |
dc.identifier.epage | 646 | - |
dc.relation.doi | 10.1016/j.bbrc.2012.05.158 | - |
dc.textversion | author | - |
dc.identifier.pmid | 22683642 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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