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dc.contributor.authorIwabuchi, Kumiko Aja
dc.contributor.authorYamakawa, Tatsuyaja
dc.contributor.authorSato, Yoshikoja
dc.contributor.authorIchisaka, Tomokoja
dc.contributor.authorTakahashi, Kazutoshija
dc.contributor.authorOkita, Keisukeja
dc.contributor.authorYamanaka, Shinyaja
dc.contributor.alternative岩渕, 久美子ja
dc.contributor.alternative山川, 達也ja
dc.contributor.alternative佐藤, 美子ja
dc.contributor.alternative一阪, 朋子ja
dc.contributor.alternative高橋, 和利ja
dc.contributor.alternative沖田, 圭介ja
dc.contributor.alternative山中, 伸弥ja
dc.description.abstractThe principal factors that lead to proliferation and pluripotency in embryonic stem cells (ESCs) have been vigorously investigated. However, the global network of factors and their full signaling cascade is still unclear. In this study, we found that ECAT11 (L1td1) is one of the ESC-associated transcripts harboring a truncated fragment of ORF-1, a component of the L1 retrotransposable element. We generated an ECAT11 knock-in mouse by replacing its coding region with green fluorescent protein. In the early stage of development, the fluorescence was observed at the inner cell mass of blastocysts and epiblasts. Despite this specific expression, ECAT11-null mice grow normally and are fertile. In addition, ECAT11 was dispensable for both the proliferation and pluripotency of ESCs.We found rapid and robust activation of ECAT11 in fibroblasts after the forced expression of transcription factors that can give rise pluripotency in somatic cells. However, iPS cells could be established from ECAT11-null fibroblasts. Our data demonstrate the dispensability of ECAT11/L1td1 in pluripotency, despite its specific expression.ja
dc.publisherPublic Library of Scienceja
dc.rights© 2011 Iwabuchi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ja
dc.subject.meshAmino Acid Sequence-
dc.subject.meshEmbryo, Mammalian/cytology-
dc.subject.meshEmbryo, Mammalian/metabolism-
dc.subject.meshEmbryonic Stem Cells/cytology-
dc.subject.meshEmbryonic Stem Cells/metabolism-
dc.subject.meshGene Expression Regulation, Developmental-
dc.subject.meshGene Knock-In Techniques-
dc.subject.meshGenes, Reporter-
dc.subject.meshGreen Fluorescent Proteins/metabolism-
dc.subject.meshInduced Pluripotent Stem Cells/cytology-
dc.subject.meshInduced Pluripotent Stem Cells/metabolism-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshPromoter Regions, Genetic/genetics-
dc.subject.meshProtein Transport-
dc.subject.meshRNA-Binding Proteins-
dc.subject.meshTranscription Factors/genetics-
dc.subject.meshTranscription Factors/metabolism-
dc.titleECAT11/L1td1 is enriched in ESCs and rapidly activated during iPSC generation, but it is dispensable for the maintenance and induction of pluripotency.ja
dc.type.niitypeJournal Articleja
dc.identifier.jtitlePloS oneja
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