このアイテムのアクセス数: 109

このアイテムのファイル:
ファイル 記述 サイズフォーマット 
j.parint.2012.07.007.pdf679 kBAdobe PDF見る/開く
タイトル: Evolution of sex chromosomes ZW of Schistosoma mansoni inferred from chromosome paint and BAC mapping analyses.
著者: Hirai, Hirohisa  kyouindb  KAKEN_id
Hirai, Yuriko
Loverde, Philip T
著者名の別形: 平井, 啓久
キーワード: Schistosoma manson
Chromosome paint
BAC mapping
FISH
Sex chromosome
発行日: Dec-2012
出版者: Elsevier Ireland Ltd.
誌名: Parasitology international
巻: 61
号: 4
開始ページ: 684
終了ページ: 689
抄録: Chromosomes of schistosome parasites among digenetic flukes have a unique evolution because they exhibit the sex chromosomes ZW, which are not found in the other groups of flukes that are hermaphrodites. We conducted molecular cytogenetic analyses for investigating the sex chromosome evolution using chromosome paint analysis and BAC clones mapping. To carry this out, we developed a technique for making paint probes of genomic DNA from a single scraped chromosome segment using a chromosome microdissection system, and a FISH mapping technique for BAC clones. Paint probes clearly identified each of the 8 pairs of chromosomes by a different fluorochrome color. Combination analysis of chromosome paint analysis with Z/W probes and chromosome mapping with 93 BAC clones revealed that the W chromosome of Schistosoma mansoni has evolved by at least four inversion events and heterochromatinization. Nine of 93 BAC clones hybridized with both the Z and W chromosomes, but the locations were different between Z and W chromosomes. The homologous regions were estimated to have moved from the original Z chromosome to the differentiated W chromosome by three inversions events that occurred before W heterohcromatinization. An inversion that was observed in the heterochromatic region of the W chromosome likely occurred after W heterochromatinization. These inversions and heterochromatinization are hypothesized to be the key factors that promoted the evolution of the W chromosome of S. mansoni.
著作権等: © 2012 Elsevier Ireland Ltd. Published by Elsevier Ireland Ltd.
この論文は著者最終稿です。内容が印刷版と異なることがありますので、引用の際には出版社版をご確認ご利用ください。This is the Accepted Author Manuscript. Please cite only the published version.
URI: http://hdl.handle.net/2433/160349
DOI(出版社版): 10.1016/j.parint.2012.07.007
PubMed ID: 22831897
出現コレクション:学術雑誌掲載論文等

アイテムの詳細レコードを表示する

Export to RefWorks


出力フォーマット 


このリポジトリに保管されているアイテムはすべて著作権により保護されています。